Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.
Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications
Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations
Covers steady-state phenomena, time-resolved phenomena, and advanced methods
Spans biophysical, biochemical, and biomedical applications
Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Für Beruf und Forschung
Professional Practice & Development
Produkt-Hinweis
Broschur/Paperback
Klebebindung
Illustrationen
109 s/w Abbildungen, 661 farbige Abbildungen
661 Illustrations, color; 109 Illustrations, black and white
Maße
Höhe: 251 mm
Breite: 178 mm
Dicke: 41 mm
Gewicht
ISBN-13
978-1-032-33737-1 (9781032337371)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Zygmunt (Karol) Gryczynski is a professor of molecular biology and immunology and the director of the Center for Commercialization of Fluorescence Technologies (CCFT) the University of North Texas Health Center.
Ignacy Gryczynski is a professor of cell biology and genetics and the director of the Microscopy Core Facility at the University of North Texas Health Center.
Autor*in
University of North Texas
University of North Texas Health Science Center, Fort Worth, USA
Preface. Theory of Light and Light Interaction with Matter. Experimental Basics. Steady State Experiments: Trasmission/Absorption. Fluorescence: Steady-State Phenomena. Steady-State Fluorescence: Applications. Steady-State Fluorescence Polarization: Anisotropy. Fluorescence: Time-Resolved Phenomena. Advanced Experiments.
