DNA Cloning: v. 1

The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries; Constructing and screening cDNA libraries in gt10 and gt11; An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors; Immunological detection of chimeric B-galactosidases expressed by plasmid vectors; The pEMBL family of single-stranded vectors; Techniques for transformation of E.Coli; The use of genetic markers for the selection and allelic exchange of in vitro induced mutations that do not have a phenotype in E. Coli; The oligonucleotide-directed construction of mutations in recombinant filamentous phage; Broad host range cloning vectors for gram negative bacteria