DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the
HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and
troubleshooting comments.
This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
halftones, line figures, tables
Maße
Höhe: 234 mm
Breite: 157 mm
Dicke: 17 mm
Gewicht
ISBN-13
978-0-19-963480-4 (9780199634804)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Department of Anatomy and PhysiologyUniversity of Dundee
Department of Biochemistry and Molecular BiologyUniversity of Leeds
1. High efficiency gene transfer into mammalian cells ; 2. Construction and characterization of Vaccinia virus recombinants ; 3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells ; 4. Retroviral vectors ; 5. Genetic manipulation of embryonic stem cells ; 6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs ; 7. Genomic and expression analysis of transgenic animals ; 8. Expression using a defective Herpes simplex virus (HSV-1) vector system ; 9. Adenovirus vectors