Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular Biology.- Maintaining Cerebellar Granule Neuron Progenitors in Cell Culture.- Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry.- Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitors.- Processing of Neonatal Mouse Brains for Immunohistochemical Analysis, As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis.- Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections.- Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections.- scRNA-seq for Microcephaly Research [1]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis.- scRNA-seq for Microcephaly Research [2]: Preparation of Single-Cell cDNA Libraries.- scRNA-seq for Microcephaly Research [3]: Computational Analysis of scRNA-seq Data.- scRNA-seq for Microcephaly Research [4]: Dirichlet Regression for Single Cell Population Differences.- Mouse Brain MRI, Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly.- Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly, using 13C Isotopes.- Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders.
