Cytoskeleton Methods and Protocols

Name: Cytoskeleton Methods and Protocols | Methods and Protocols
Brand: Humana Press Inc.
Price: 85.59 EUR
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Methods and Protocols

Ray H. Gavin(Herausgeber*in)

Humana Press Inc.

Erschienen am 1. Januar 2001

Buch

Hardcover

XIV, 288 Seiten

978-0-89603-771-7 (ISBN)

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Beschreibung

Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments. Rather than a network of disparate fibers, these polymers are often interconnected and display synergy, which is the combined action of two or more cytoskeletal polymers to achieve a specific cellular structure or function. Cross-commu- cation among cytoskeletal polymers is thought to be achieved through cytoskeletal polymer accessory proteins and molecular motors that bind two or more cytoskeletal polymers. Development of the modern concept of the cytoskeleton is a direct o- growth of advances in experimental tools and reagents that are available to cell and molecular biologists. Technological advances and refinements in cell imaging have made it possible to selectively image a single cytoskeletal po- mer and monitor its dynamics through the use of fluorescence probes in vitro and in vivo. Two decades ago, cytoskeletal research was limited to a few perturbation reagents that included colchicine and cytochalasin. Today, the perturbation arsenal has expanded to a highly selective group of reagents that includes Taxol, nocodazole, benomyl, latrunculin, jasplakinolide, and such endogenous proteins as gelsolin. These reagents enable the investigator to selectively perturb or destroy a cytoskeletal polymer while leaving other cytoskeletal polymers intact. Site-specific monoclonal antibodies that target a specific cytoskeletal polymer have proven to be highly selective affinity tools for cytoskeletal research.

Rezensionen / Stimmen

The authors of the chapters are highly experienced and widely recognized as experts in their fields The volume is one that the libraries of many universities and research institutes will want to own as a resource for their cytoskeleton researchers. - TRENDS in Cell Biology The material and procedures sections of the chapters are generally well detailed such that one with little previous experience might be able to carry out the procedure or adapt it to one's own particular system. The chapters include helpful hints on the techniques which are often not found in published articles. As a microscopist, I was impressed by the simple but elegant procedure of placing a finger print on the corner of a cover slip in order to determine the plane of focus in video-enhanced microscopy, presented by George Langford in Chapter 4. It is often just those very kinds of things that can mean the difference between success and failure, or at least help to reduce the frustration level of a first timer. - Microscopy and Microanalysis As a cell biologist, I was impressed by the chapters dealing with the study of cytoskeletal dynamics in living cells using green fluorescent protein and the study of organelle transport using chromatophores as tools you got the true impression you can do it thanks to the step-by-step protocols, you feel you cannot be wrong and I personally worked out one clear experiment. I will do on my own on mouse oocytes. I think the whole scientific community has to thank Dr. Gavin for providing us with such a helpful book. European Journal of Histochemistry Cytoskeleton: Methods and Protocols emphasizes an integrative approach and is intended for novice and advanced researchers. It is highly recommended for libraries serving researchers interested in cytoskeleton structure and function and related areas of cell biology. E-STREAMS

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Inhalt

Part I. Identification of Cytoskeleton Proteins. Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments, Jorge A. Garcés and Ray H. Gavin. Microsequencing of Myosins for PCR Primer Design, Elaine L. Bearer. Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena,Gangadhara Sailaja, Leslie M. Lincoln, Jifan Chen, and David J. Asai. Part II. Microscopy Applications. Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function, George M. Langford. Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility, David R. Soll, Deborah Wessels, Edward Voss, and Olof Johnson. Evaluation of Individual-Cell Motility, Peter S. Walmod, Rasmus Hartmann-Petersen, Anton Berezin, Søren Prag, Vladislav V. Kiselyov, Vladimir Berezin, and Elizabeth Bock. Evaluation of Cell Morphology, Eugene A. Lepekhin, Peter S. Walmod, Anton Berezin, Vladimir Berezin, and Elisabeth Bock. A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena, Uffe Koppelhus, Per Hellung-Larsen, and Vagn Leick. Part III. Reagents for Studying Cytoskeleton Protein Function. Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization, Andreas Holzinger. Site-Directed Antibodies as Tools For Investigating Structure and Function of Cytoskeleton Proteins, Walter Steffen and Julie L. Hodgkinson. Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy, Julie L. Hodgkinson and Walter Steffen. Tat-Mediated Delivery of Antibodies into Cultured Cells, Walter Steffen. Part IV. Cytoskeleton Dynamics. Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein, Yisang Yoon, Kelly R. Pitts, and Mark A. McNiven. Use of Green Fluorescent Protein to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enzymes, Hong Cao, Heather M. Thompson, Eugene W. Krueger, and Mark A. McNiven. Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics, Jesús M. Paramio and José L.Jorcano. Part V. Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function. Chromatophores as Tools for the Study of Organelle Transport, Bruce R. Telzer and Leah T. Haimo. Centriole Duplication, Centrosome Maturation, and Spindle Assembly in Lysates of Spisula solidissima Oocytes, Andrew W. Suddith, Eugeni A. Vaisberg, Sergei A. Kuznetsov, Walter Steffen, Conly L. Rieder, and Robert E. Palazzo. Xenopus Egg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate Filament Interactions, Craig A. Mandato, Kari L. Weber, Anna J. Zandy, Thomas J. Keating, and William M. Bement. Detergent-Extracted Models for the Study of Cilia or Flagella, Charles B. Lindemann and Kathleen A. Schmitz. Part VI. Genetic Approaches for Studies of Cytoskeleton Protein Function. A Yeast Two-Hybrid Approach for Probing Cytoskeletal Protein Interactions, Jin-jun Meng and Wallace Ip. Manipulating Dynein Genes in Tetrahymena, David J. Asai, K. Mark DeWall, Leslie M. Lincoln, and Renotta K. Smith. Functional Analysis of Cytoskeletal Components in the Developing Visual System of Drosophila, Qi He. Index.

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