Cellular Spatial Characterization and Analysis

1. High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).- Multiplex Immunofluorescence Staining of Paraffin-Embedded Tissue: Coverslip Mounting of Tissue Sections Improves Z-axis Optical Resolution in Widefield Microscopy.- Evaluation of Murine Tissue Architecture and Cellular Spatial Relationships in Murine Tumor Microenvironments Utilizing Multiplex Fluorescent Immunohistochemistry.- Autostaining Immune Multiplex Immunohistochemistry Panels and Imaging Analysis.- Fully Integrated Sample Preparation-Based Spatial Proteomic Analysis.- Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy.- Current Methods in CODEX High-Multiplex Microscopy to Describe Spatial Relationships in the Tissue Microenvironment.- Mapping the Tumor Microenvironment with Integrative Single-Cell RNA Sequencing and Spatial Proteomics: Uncovering Mechanisms of Disease and Therapeutic Resistance.- Single-Molecule Fluorescence In Situ Hybridization (smFISH) for RNA Localization Relative to a DNA Locus in Bacteria.- A Protocol for Spatial Transcriptomics using the 10x Genomics Visium Platform.- Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.- From Raw Data to Biological Insights: A Practical Guide for Spatial Transcriptomics Analysis in R and Python.