Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Auflage
Sprache
Verlagsort
Zielgruppe
Illustrationen
71 halftones, 55 line drawings
Maße
Höhe: 234 mm
Breite: 156 mm
Dicke: 23 mm
Gewicht
ISBN-13
978-0-19-518218-7 (9780195182187)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Joachim Frank is an Investigator at the Howard Hughes Medical Institute. He is also Professor of Biomedical Sciences and Adjunct Professor of Biology at the State University of New York at Albany, Adjunct Professor of Biochemistry and Molecular Biophysics at Columbia University, Research Professor of Cell Biology at New York State University Medical School, and head of the Laboratory of Computational Biology and Macromolecular Imaging at Health
Research, Inc., the Wadsworth Center.
Autor*in
Investigator, Laboratory of Computational Biology and Molecular ImagingInvestigator, Laboratory of Computational Biology and Molecular Imaging, Howard Hughes Medical Institute
1: Introduction
2: Electron Microscopy of Macromolecular Assemblies
3: Two-Dimensional Averaging Techniques
4: Multivariate Data Analysis and Classification of Images
5: Three-Dimensional Reconstruction
6: Interpretation of Three-Dimensional Images of Macromolecules
Appendix 1: Some Important Definitions and Theorems
Appendix 2: Profiles, Point-Spread Functions, and Effects of Commonly Used Low-Pass Filters
Appendix 2: Bibliography of Methods
Appendix 2: Bibliography of Structures
Appendix 2: Special Journal Issues on Image Processing Techniques
