Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects.
Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization.
Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.
Rezensionen / Stimmen
From the reviews:
"A compendium of techniques and applications that will be of wide utility to researchers interested in gene identification and function. . this volume will be of interest to a broad audience of molecular biologists, geneticists and biochemists and will provide a valuable resource in the laboratory due to its applicability to a wide breadth of projects. . for labs with a strong interest in proteomics and genomics it would certainly be well worth buying a copy to share. . I would certainly recommend this book." (Barbara Guinn, Immunology News, May, 2008)
Reihe
Auflage
Sprache
Verlagsort
Zielgruppe
Für Beruf und Forschung
Research
Illustrationen
60
60 s/w Abbildungen
XVI, 358 p. 60 illus.
Maße
Höhe: 241 mm
Breite: 160 mm
Dicke: 25 mm
Gewicht
ISBN-13
978-1-58829-504-0 (9781588295040)
DOI
10.1007/978-1-59745-335-6
Schweitzer Klassifikation
Reciprocal Subtraction Differential RNA Display (RSDD).- Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH).- The Application of Differential Display as a Gene Profiling Tool.- Serial Analysis of Gene Expression (SAGE).- Gene Expression Profile Analysis of Tumors.- cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-?B Related Genes in Squamous Cell Carcinoma.- Gene Profiling Uncovers Retinoid Target Genes.- Complete Open Reading Frame (C-ORF) Technique.- Chromatin Immunoprecipitation Assays.- Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection.- Molecular Cytogenetic Applications in Analysis of the Cancer Genome.- Monitoring Methylation and Gene Expression in Cancer.- The Use of Phage Display Peptide Libraries for Basic and Translational Research.- Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions.- Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated With Tumor Growth Arrest.- Surface-Epitope Masking (SEM).- Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells.- PKR in Innate Immunity, Cancer, and Viral Oncolysis.- Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells.- Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells.- Reverse Phase Protein Microarrays for Monitoring Biological Responses.- Protein Crystallization.
