This volume comprises basic protocols by experts which aim to provide a broad spectrum of easily reproducible methods for analyzing gene, mRNA, protein expression, as well as the function of cytokines and cytokine receptors. The protocols use a variety of model systems and types of samples and include some on gene polymorphism, ligand-receptor interactions, signalling routes and soluble cytokine binding proteins. Strategies for the search of new cytokine(receptor) genes, purification and characterization of cytokine (receptors), design of cytokine antagonists, and the use of cytokine antagonists to modulate cytokine activity are also presented.
This volume comprises basic protocols by experts which aim to provide a broad spectrum of easily reproducible methods for analyzing gene, mRNA, protein expression, as well as the function of cytokines and cytokine receptors. The protocols use a variety of model systems and types of samples and include some on gene polymorphism, ligand-receptor interactions, signalling routes and soluble cytokine binding proteins. Strategies for the search of new cytokine(receptor) genes, purification and characterization of cytokine (receptors), design of cytokine antagonists, and the use of cytokine antagonists to modulate cytokine activity are also presented.
Reihe
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Maße
Höhe: 229 mm
Breite: 153 mm
Gewicht
ISBN-13
978-0-89603-445-7 (9780896034457)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
Introduction to Cytokines and Cytokines Receptors R. Debets and H. Savelkoul. Cytokine DNA and Messenger RNA. Search for Novel Genes Using Degenerate Oligonucleotide PCR K. Yamamoto. Detection and Analysis of Polymorphisms G. Duff. In Situ Hybridization of mRNA R. Debets. Competitive Reverse Transcription-Polymerase Chain Reaction to Quantify Small Amounts of mRNA H-Q. Xiao G. Krisnaswamy S-K. Huang and M. Liu. Cytokine Protein. Chromatographical Purification R. Kastelein. Electrophoretic Analysis R. Kastelein. Recombinant Antibodies from Phage Display Libraries J. De Kruiff J. Van Der Heijden and T. Logtenberg. Immunoassays M. Laan W. Buurman and H. Savelkoul. Immunolocalization of Cytokines E. Minshall and Q. Hamid. Cytoplasmic Cytokine Staining in Individual Cells M. Litton J. Andersson L. Bjrk T. Fehniger A-K. Ulfgren and U. Andersson. Secretion of Cytokines by Individual Cells B. Bast and W. Vooys. Structure - Function Analysis of Cytokines M. Ehlers and S. Rose-John. Cytokine Receptors. Receptor Characterization A. Shanafelt. Flow Cytometric Analysis of Binding Sites J. Hegmans and E. Prens. Biosensor Analysis of Receptor-Ligand Interactions P. Edwards. Structure-Function Analysis of Receptors C. Hebert. Analysis of Soluble Receptors P. Vollmer and S. Rose-John. Cytokine Signaling. Assays for Second Messengers R. Kastelein. Analysis of Phosphorylated Intracellular Substrates J. Borst. Analysis of Activated Transcription Factors N. Danial and P. Rothman. Cytokine Activity. Assays for Antiviral Activity A. Osterhaus Assays for Human Cell-Mediated Cytoxicity E. Grimm. In Vitro and In Vivo Test for Leucocyte Migration A. Wuyts P. Proost A. Haelens S. Struyf J-P. Lenaerts N. Van Osselaer and J. Van Damme. Assay For Leucocyte Adhesion and Transendothelial Migration P. Allavena B. Giancarlo and A. Mantovani. Serum-Free Liquid Culture Assay of Maintenance and Generation of Human Stem Cell Subsets R. Ploemacher and D. Breems Assays for Proliferation M. Topp. Monocyte or Cytokine Induced Apoptosis: A Flow-Cytometric Assay A. Van De Loosdrecht G. Ossenkoppele and M. Langenhuijsen. Quantitative Biological Assays Using Cytokine-Dependent Cell-Lines A. Mire-Sluis and R. Thorpe. Modulation of Cytokine Activity by Cytokine Antagonists. Modulation of Human Interferon-g Biosynthesis by Antisense Oligodeoxynucleotides C. Boeve and M. De Ley. Autoantibodies to Cytokines K. Bendtzen M. Hansen C. Ross and M. Svenson. Muteins to Inhibit Receptor Binding or Activation T. Mueller W. Sebald and H. Oschkinat. Identification of the Molecular Target of Selective Protein Kinase Inhibitors Using Radio-Ligand Binding and Photo- Affinity Labeling M. Blumenthal J. Laydon and J. Lee. Selective Inhibition of MAP Kinase Activity S. Kumar M. Blumenthal P. Young and J. Lee. Recent Viewpoints. Activation of Cytokine Genes in T Cells H. Savelkoul. Induction of Polarized Cytokine Profiles in CD4+ Helper T Cells D. Umetsu and R. De Kruyff. Paradoxical Molecular Regulation of Soluble Cytokine in Receptors Human Sepsis Syndrome J. Christman and T. Blackwell. Index.
