Equine Reproductive Procedures is a user-friendly guide to reproductive management, diagnostic techniques, and therapeutic techniques on stallions, mares, and foals. Offering detailed descriptions of 161 procedures ranging from common to highly specialized, the book gives step-by-step instructions with interpretative information, as well as useful equipment lists and references for further reading. Presented in a highly portable spiral-bound format, Equine Reproductive Procedures is a practical resource for daily use in equine practice.
Divided into sections on the non-pregnant mare, the pregnant mare, the postpartum mare, the stallion, and the newborn foal, the book is well-illustrated throughout with clinical photographs demonstrating procedures. Equine Reproductive Procedures provides practical guidance for performing basic and advanced techniques associated with the medical management of horses.
Rezensionen / Stimmen
"However, these rare omissions and errors should be overlooked because this book is a brilliant addition to the library of any equine practitioner involved in the reproductive management of horses." (JAVMA, 15 December 2014)
Auflage
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Maße
Höhe: 24.3 cm
Breite: 20.9 cm
Dicke: 2.9 cm
Gewicht
ISBN-13
978-0-470-96039-4 (9780470960394)
Schweitzer Klassifikation
John Dascanio, VMD, DACT, DABVP (Equine), was formerly a Professor at Ross University School of Veterinary Medicine in Basseterre, St. Kitts, West Indies, and is currently a Professor at Lincoln Memorial University - College of Veterinary Medicine in Harrogate, Tennessee, USA.
Patrick McCue, DVM, PhD, Diplomate ACT, is the Iron Rose Ranch Professor of Equine Theriogenology at Colorado State University in Fort Collins, Colorado, USA.
Herausgeber*in
Lincoln Memorial University - College of Veterinary Medicine in Harrogate, Tennessee, USA
Colorado State University, Fort Collins, Colorado, USA
List of Contributors xiii
Preface xv
Section I Non-Pregnant Mare
1 Reproductive Evaluation of the Mare 3
Patrick M. McCue
2 Teasing 6
Patrick M. McCue
3 TailWrap and Preparation/Washing of the Perineum 9
John J. Dascanio
4 Placement of a Tail Rope 14
John J. Dascanio
5 Perineal Conformation Evaluation 17
John J. Dascanio
6 Removal of a Persistent Hymen 20
John J. Dascanio
7 Palpation of the Reproductive Tract of the Non-Pregnant Mare 22
Patrick M. McCue
8 Ultrasound Evaluation of the Non-Pregnant Mare 26
Patrick M. McCue
9 Prediction of Ovulation 32
Patrick M. McCue
10 Speculum Examination of the Vagina 35
John J. Dascanio
11 Digital Examination of the Vagina/Cervix 38
Sofie Sitters
12 Uterine Culture Collection: Swab/Brush 41
John J. Dascanio
13 Antimicrobiotic Sensitivity Testing 44
Jillian Bishop and Patrick M. McCue
14 Microbiology: Microbial Culture 46
Patrick M. McCue and Jillian Bishop
15 Microbiology: Gram Stain 51
Jillian Bishop and Patrick M. McCue
16 qPCR Assay for the Diagnosis of Infectious Endometritis 53
Ryan A. Ferris and Patrick M. McCue
17 Uterine Cytology Collection: Swab/Brush 58
John J. Dascanio and Ryan A. Ferris
18 Uterine Culture/Cytology: Low Volume Lavage 64
John J. Dascanio
19 Endometrial Biopsy 68
Patrick M. McCue
20 Hysteroscopic Examination of the Uterus 71
Patrick M. McCue
21 Endometrial Cyst Removal 74
John J. Dascanio
22 Chromosomal Analysis 77
Teri L. Lear
23 Endocrinological Examination 83
Patrick M. McCue
24 Laparoscopic Examination of the Uterus and Ovaries 85
Fausto Bellezzo
25 Evaluation of the Mammary Gland 88
Patrick M. McCue
26 Antisperm Antibody Testing 91
Patrick M. McCue
27 Starch Granule Test for the Evaluation of Oviductal Patency 93
Sofie Sitters and John J. Dascanio
28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 95
Sofie Sitters and John J. Dascanio
29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 97
Sofie Sitters and Patrick M. McCue
30 Prostaglandin E2 Treatment for Blocked Oviducts 100
Patrick M. McCue and Sofie Sitters
31 Uterine Lavage 102
John J. Dascanio
32 Uterine Infusion 106
John J. Dascanio
33 Restraint for Breeding 109
John J. Dascanio
34 Natural Service: Pasture Breeding 112
Patrick M. McCue
35 Natural Service: In-hand Breeding 114
Charles F. Scoggin
36 Breeding Stitches 117
Charles F. Scoggin
37 Reinforcement Breeding 119
Charles F. Scoggin
38 Breeding with Fresh or Cooled Semen 121
John J. Dascanio and Patrick M. McCue
39 Insemination Through a Vaginal Speculum 125
Patrick M. McCue
40 Breeding with Frozen Semen 127
John J. Dascanio
41 Deep Horn Insemination 131
John J. Dascanio
42 Hysteroscopic (Low Dose) Insemination 133
Patrick M. McCue
43 Caslick Operation or Vulvoplasty 136
John J. Dascanio
44 Contagious Equine Metritis Testing 140
John J. Dascanio
45 Management of Seasonal Anestrus: Artificial Photoperiod 144
Patrick M. McCue
46 Management of Seasonal Anestrus: Hormone Therapy 146
Patrick M. McCue
47 Hormone Therapy in Cycling Mares 149
Patrick M. McCue
48 Estrus Suppression 155
Patrick M. McCue
49 Use of an Intrauterine Ball in Estrus Suppression 158
John J. Dascanio
50 Harvesting and Shipping Ovaries for Oocyte Recovery 161
Patrick M. McCue
Section II Pregnant Mare
51 Embryo Collection 165
Patrick M. McCue
52 Embryo Evaluation 169
Patrick M. McCue
53 Embryo Biopsy 173
Patrick M. McCue
54 Embryo Packaging for Cooled Transport 175
Patrick M. McCue
55 Embryo Cryopreservation 178
Patrick M. McCue
56 Non-Surgical Embryo Transfer 182
Patrick M. McCue
57 Palpation of the Pregnant Mare Per Rectum 185
Sofie Sitters
58 Ultrasound Examination of the Pregnant Mare 188
Patrick M. McCue
59 Speculum Examination of the Pregnant Mare 193
Sofie Sitters
60 Ultrasound Determination of Fetal Sex 195
Richard D. Holder
61 Assessment of Fetal Well-being 201
John J. Dascanio
62 Combined Thickness of the Uterus and Placenta 205
Patrick M. McCue
63 Doppler Ultrasonography 208
John J. Dascanio
64 Prediction of Fetal Age 212
John J. Dascanio
65 Twin Reduction: Manual Technique 216
John J. Dascanio
66 Twin Reduction: Transvaginal Aspiration 219
John J. Dascanio
67 Twin Reduction: Cranio-Cervical Dislocation 222
Sofie Sitters and Karen Wolfsdorf
68 Twin Reduction: Transabdominal Fetal Cardiac Puncture 226
John J. Dascanio
69 Elective Termination of Pregnancy 229
John J. Dascanio
70 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 232
Julie T. Cecere and John J. Dascanio
71 Endocrine Evaluation of Pregnancy 235
Patrick M. McCue
72 Treatment of Vaginal Varicosities 240
John J. Dascanio
73 Manual Correction of Uterine Torsion 243
John J. Dascanio
74 Preparation of the Mare for Foaling 246
John J. Dascanio
75 Assessment of Mammary Gland Secretions to Predict Foaling 249
John J. Dascanio
76 Assessment of pH of Mammary Gland Secretions to Predict Foaling 253
Patrick M. McCue
77 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 256
Ryan A. Ferris and Etta A. Bradecamp
78 Foaling Alert: Vulvar Device 259
John J. Dascanio
79 Positional Labor Alert Devices 261
Etta A. Bradecamp and Ryan A. Ferris
80 Induction of Parturition 264
John J. Dascanio
81 Premature Separation of the Placenta 267
Patrick M. McCue
82 Dystocia Management 270
Patrick M. McCue and Sofie Sitters
83 Dystocia Correction 275
John J. Dascanio
84 Fetotomy 280
John J. Dascanio
85 Epidural 285
John J. Dascanio
86 Induction of Lactation to Create a Nurse Mare 287
Patrick M. McCue
87 Screening the Pregnant Mare to Prevent Neonatal Isoerythrolysis 289
Patrick M. McCue
Section III Postpartum Mare
88 Evaluation of Colostrum Specific Gravity 293
Patrick M. McCue
89 Evaluation of Colostrum Quality: Brix Refractometry 297
Patrick M. McCue
90 Colostrum Banking 299
Patrick M. McCue
91 Obtaining Milk from the Mare 302
John J. Dascanio
92 Placental Evaluation 304
Patrick M. McCue
93 Removal of Retained Placenta 309
John J. Dascanio
94 Abdominocentesis in the Postpartum Mare 312
John J. Dascanio
95 Uterine Prolapse Treatment 314
John J. Dascanio
96 Buhner Needle Placement of a Perivulvar Suture 317
John J. Dascanio
Section IV Stallion
97 Breeding Soundness Evaluation of the Stallion 321
Patrick M. McCue
98 Training of a Stallion to Use a Phantom 325
John J. Dascanio
99 Missouri Artificial Vagina 329
John J. Dascanio
100 Colorado Model Artificial Vagina 332
Patrick M. McCue
101 Roanoke Artificial Vagina 336
John J. Dascanio
102 Hannover Artificial Vagina 340
Sofie Sitters
103 Teaser Stallions 342
Charles F. Scoggin
104 Standing Semen Collection 344
John J. Dascanio
105 Chemical Ejaculation 347
Patrick M. McCue
106 Preparation of a Jump Mare or a Mare for Natural Cover 349
John J. Dascanio
107 Washing the Penis 351
John J. Dascanio
108 Evaluation of Sexual Behavior in the Stallion 354
John J. Dascanio
109 Calibrated Spectrophotometer Evaluation of Sperm Concentration 357
John J. Dascanio
110 Hemocytometer Evaluation of Sperm Concentration 360
John J. Dascanio
111 NucleoCounter(r) Evaluation of Sperm Concentration and Viability 363
Patrick M. McCue
112 Visual Evaluation of Sperm Motility 366
Patrick M. McCue
113 Computer-Assisted Sperm Analysis 368
John J. Dascanio
114 Eosin-Nigrosin Staining in the Evaluation of Sperm 373
Julie T. Cecere
115 Evaluation of Sperm Morphology 377
Leonardo Brito
116 Determination of Daily Sperm Output 384
Patrick M. McCue
117 Measurement of Testicular Size and Estimation of Daily Sperm Output 386
Patrick M. McCue
118 Wet Mount Evaluation of Sperm 389
John J. Dascanio
119 Diff-Quik(r) Evaluation of Round Cells and Sperm 392
John J. Dascanio
120 Electron Microscopy of Semen 394
D. N. Rao Veeramachaneni
121 Bacterial Culture in the Stallion 396
John J. Dascanio
122 Evaluation of pH and Osmolarity of Semen 399
Patrick M. McCue
123 Alkaline Phosphatase: A Marker for Ejaculation 401
Patrick M. McCue
124 Diagnosis and Management of Urospermia 403
John J. Dascanio
125 Semen Extenders and Sperm Media 406
Patrick M. McCue
126 Preparation of Semen for Cooled Transport 409
Etta A. Bradecamp
127 Packing Semen for Cooled Transport 412
Etta A. Bradecamp
128 Longevity Testing of Sperm 419
John J. Dascanio
129 Calculation of g Force for Centrifuging Semen 421
John J. Dascanio
130 Centrifugation of Semen: Standard Technique 424
Etta A. Bradecamp
131 Centrifugation of Semen: Cushion Technique 429
Etta A. Bradecamp
132 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 433
Etta A. Bradecamp
133 Semen Freezing 436
Patrick M. McCue
134 Thawing Frozen Semen 441
John J. Dascanio
135 Preparing a Vapor Shipper 444
Julie T. Cecere
136 Loading a Vapor Shipper 448
Julie T. Cecere, Patrick M. McCue, and John J. Dascanio
137 Maintaining a Long-Term Frozen Semen Storage Tank 450
John J. Dascanio
138 Harvesting and Shipping Testes 453
John J. Dascanio
139 Epididymal Sperm Recovery 455
John J. Dascanio
140 Endoscopic Examination of the Urethra 458
Patrick M. McCue
141 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 461
Sofie Sitters
142 Palpation and Ultrasonography of the Accessory Sex Glands 466
Sofie Sitters
143 Radiographic Examination of the Penis 471
Patrick M. McCue
144 Testicular Biopsy and Aspiration 473
John J. Dascanio
145 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 476
Amanda I. Glazar
146 Sperm Chromatin Structure Assay 478
Charles Love
147 Hypo-Osmotic Swelling Test 481
Patrick M. McCue
148 Assessment of Sperm Acrosomal Status: FITC PNA 483
Amanda I. Glazar
149 Assessment of Sperm Mitochondrial Function: JC-1 and Rhodamine 123 486
Amanda I. Glazar
150 Equine Viral Arteritis Testing 488
G. Reed Holyoak
151 Diagnostic Endocrinology: Baseline Hormone Levels 492
Patrick M. McCue
152 Diagnostic Endocrinology: GnRH Stimulation Tests 494
Patrick M. McCue
153 Diagnostic Endocrinology: hCG Stimulation Test 496
Patrick M. McCue
154 Diagnostic Endocrinology: Estrogen Conjugate Assay 499
Patrick M. McCue
155 Probang Apparatus 501
Julie T. Cecere
156 Support Apparatus for Paraphimosis 504
John J. Dascanio
Section V Newborn Foal
157 Birth Resuscitation 511
Patrick M. McCue
158 Evaluation of Passive Transfer 514
Patrick M. McCue
159 Colostral Administration via Naso-Gastric Intubation 518
John J. Dascanio
160 Routine Care 521
Patrick M. McCue
161 Foal Rejection 527
Patrick M. McCue
Section VI Appendices
Appendix 1 Society for Theriogenology Stallion Reproductive Evaluation Form 533
Appendix 2 Mare Breeding Soundness Evaluation Form 537
Appendix 3 Formulary for Equine Reproduction 540
Appendix 4 Foaling Kit and Associated Equipment and Supplies 549
Index 551
