Gene probes, whether RNA or DNA, have played a central role in the rapid development of molecular biology. The wide variety of applications is matched by a considerable diversity in the methods used for generating probes. A complete account of the available methods and their applications is an almost impossible task. Instead the editors of this series have attempted a compromise by combining a selection of newer procedures with a review of the most important established methods, together with some examples of the ways in which gene probes can be applied. By doing so, the aim is to provide not only an introductory manual that will be of benefit to newcomers to the field, but also to broaden the horizons of existing researchers. Among the themes explored in this inaugural volume are the generation of labelled probes, hybridization and the uses and methods of detection of isotopic and non-isotopic probes.
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Technology
Zielgruppe
Illustrationen
line and b&w illustrations, index
Gewicht
ISBN-13
978-1-55938-263-2 (9781559382632)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Molecular Biology Group, Department of Microbiology, University of Surrey, Guildford, England
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England
Contents. List of Contributors. Introduction. Principles of Nucleic Acid Hybridization (J.J. McFadden). Basic Procedures for 5'- and 3'-End Labeling and Generating cDNA Probes (P.G. Sanders and J.W. Dale). Synthesis of RNA Probes Using SP6, T7 and T3 RNA Polymerase (P.A. Krieg). Labeling of Probes by the Random Primer Procedure (A.P. Feinberg). Film Detection Procedures for Radioactive Isotopes (W.M. Bonner). Non-Isotopic Labeling and Detection (G.R. Coulton and A.D.B. Malcolm). Photobiotin Labeling of DNA and RNA Hybridization Probes (J.L. McInnes and R.H. Symons). Nucleic Acid Hybridizations with Positive Charge-Modified Nylon Membrane (K.C. Reed). Plaque and Colony Screening Using Nucleic Acid Probes (K.J.H. Robson). In Situ Hybridization (A.J. Easton and R.P. Eglin). Characterization of mRNA Transcripts by S1 Mapping and Primer Extension (J.N. Hansen and S. Banerjee). The Polymerase Chain Reaction: Basic Procedures and Use for Virus Detection (J.P. Clewley). The Use of Polymerase Chain Reaction for cDNA Synthesis (T.M.J. Leu, C.C. Kuenzle, and M.W. Berchtold). RFLPs: Uses and Methods of Detection (K. Johnson and E. Nimmo). Transcription-Based Amplification System (D.Y. Kwoh, T.J. Kwoh, and T.R. Gingeras). Index.
