From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition
Jeremy W. Dale and Malcolm von Schantz, University of Surrey, UK.
Rapid advances in a collection of techniques referred to as gene technology, genetic engineering, recombinant DNA technology and gene cloning have pushed molecular biology to the forefront of the biological sciences. From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition explains key ideas underlying the most central techniques in the context of the ways in which they are used. As well as an overall update of the material, this new edition includes the strengthening of the gene to genome theme, additional emphasis on proteomics, gene therapy and stem cells, and more eukaryotic/mammalian examples.
Opening with a brief review of the basic concepts of molecular biology, followed by a description of the key molecular methods and how they fit together, the book progresses from the cloning, characterisation and manipulation of individual genes through to genome sequence and analysis, and the genome-wide study of transcription and translation. Finally, the book introduces the use of these techniques for genetic modification of organisms, ranging from live vaccines to transgenic plants and animals, including the prospects for gene therapy.
* An accessible introduction to the key concepts and techniques in an exciting and rapidly developing field.
* Includes basic concepts as well as the latest developments.
* Introduces selected aspects of bioinformatics.
* Clear presentation with two-colour design throughout.
The new edition of this concise, clearly written textbook will prove invaluable to those studying intermediate molecular genetics within the biological and biomedical sciences as well as established researchers needing to update their knowledge of this fast moving field.
Rezensionen / Stimmen
"... an excellent book... achieves all of its goals with style, clarity and completeness... You can see the power and possibilities of molecular genetics as you read..." -Human Genetics "This volume hits an outstanding balance among readability, coverage, and detail." -Biochemistry and Molecular Biology Education
Auflage
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Editions-Typ
Illustrationen
Maße
Höhe: 24.4 cm
Breite: 16.8 cm
Dicke: 21 mm
Gewicht
ISBN-13
978-0-470-01734-0 (9780470017340)
Schweitzer Klassifikation
Professor Jeremy Dale and Dr Malcolm von Schantz. School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 SXH, UK.
Preface
1 Introduction
2 Basic molecular biology
2.1 Nucleic acid structure
2.2 What is a gene?
2.3 Information flow: gene expression
2.4 Gene structure and organization
3 How to clone a gene
3.1 What is cloning?
3.2 Overview of the procedures
3.3 Gene libraries
3.4 Hybridization
3.5 Polymerase chain reaction
3.6 Extraction and purification of nucleic acids
3.7 Detection and quantitation of nucleic acids
3.8 Gel electrophoresis
4 Cutting and joining DNA
4.1 Restriction endonucleases
4.2 Ligation
4.3 Modification of restriction fragment ends
4.4 Other ways of joining DNA molecules
5 Vectors
5.1 Plasmid vectors
5.2 Vectors based on the lambda bacteriophage
5.3 Cosmids
5.4 M13 vectors
CONTENTS
5.5 Expression vectors
5.6 Vectors for cloning and expression in eukaryotic cells
5.7 Supervectors: YACs and BACs
5.8 Summary
6 Genomic and cDNA libraries
6.1 Genomic libraries
6.2 Growing and storing libraries
6.3 cDNA libraries
6.4 Random, arrayed and ordered libraries
7 Finding the right clone
7.1 Screening libraries with gene probes
7.2 Screening expression libraries with antibodies
7.3 Subcloning
7.4 Characterization of plasmid clones
8 Polymerase chain reaction
8.1 The PCR reaction
8.2 PCR in practice
8.3 Cloning PCR products
8.4 Long-range PCR
8.5 Reverse-transcription PCR
8.6 Rapid amplification of cDNA ends
8.7 Quantitative PCR
8.8 Applications of PCR
9 Characterization of a cloned gene
9.1 DNA sequencing
9.2 Databank entries and annotation
9.3 Sequence analysis
9.4 Sequence comparisons
9.5 Protein structure
9.6 Confirming gene function
10 Analysis of gene expression
10.1 Analysing transcription
10.2 Methods for studying the promoter
10.3 Regulatory elements and DNA-binding proteins
10.4 Translational analysis
CONTENTS
11 Products from native and manipulated cloned genes
11.1 Factors affecting expression of cloned genes
11.2 Expression of cloned genes in bacteria
11.3 Expression in eukaryotic host cells
11.4 Adding tags and signals
11.5 In vitro mutagenesis
11.6 Vaccines
12 Genomic analysis
12.1 Genome sequencing
12.2 Analysis and annotation
12.3 Comparing genomes
12.4 Genome browsers
12.5 Relating genes and functions: genetic and physical maps
12.6 Transposon mutagenesis and other screening techniques
12.7 Conclusion
13 Analysis of genetic variation
13.1 Single nucleotide polymorphisms
13.2 Larger-scale variations
13.3 Other methods for studying variation
13.4 Human genetic diseases
13.5 Molecular phylogeny
14 Post-genomic analysis
14.1 Analysing transcription; transcriptomes
14.2 Array-based methods
14.3 Translational analysis; proteomics 3
14.4 Post-translational analysis: protein interactions
14.5 Integrative studies; systems biology
15 Modifying organisms; transgenics
15.1 Modification of bacteria and viruses: live vaccines
15.2 Transgenesis and cloning
15.3 Animal transgenesis
15.4 Applications of transgenic animals
15.5 Transgenic plants and their applications
Glossary
Bibliography
Index
