Immunohistochemical techniques have had a profound influence on the development of neurobiology in recent decades. In this second edition, international researchers describe both novel and established methods and provide interpretations of the results and validity of the various techniques used, particularly when applied for the investigation of the nervous system. Main topics include antibody specificity, immunogens, production, preparation and special immunocytochemistry, the preparation and use of enzyme and gold labelled antibodies, and immunohistochemistry in vitro.
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Für Beruf und Forschung
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Höhe: 245 mm
Breite: 184 mm
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ISBN-13
978-0-471-93460-8 (9780471934608)
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Schweitzer Klassifikation
Use of Synthetic Peptide Determinants for the Production of Antibodies; Antibody Specificity in Immunocytochemistry; Preparation and Application of Conventional and Non-Conventional Monoclonal Antibodies; Current Protocols for Light Microscopy Immunocytochemistry; The Use of Avidin-Biotin Interaction in Immunocytochemistry; Pre-Embedding Electron Microscopic Immunocytochemistry; Post-Embedding Immunogold Staining; Electron Microscopic Labelling of Endogenous and/or Transported Antigens in Rat Brain Using Silver Intensified One Nanometer Colloidal Gold Conjugated Immunoglobulins; A Review of in situ Hybridization Technique: Relevance to Combined Immunocytochemical Studies; Retrograde Neuronal Tracing Combined with Immunocytochemistry; Anterograde PHAL-Tracing Combined with Pre- and Post-Embedding Immunocytochemistry; Golgi Impregnation Combined With Pre-And Post-Embedding Immunocytochemistry; Ultrastructural Immunocytochemistry Combined with Intracellular Marking of Physiologically Identified Neurons in vivo; Immunocytochemical Applications of Confocal Microscopy; Immunocytochemistry of Second Messenger Molecule; The Study of Formaldehyde-Fixed Cyclic GMP.
