1. Introduction (what the book will teach you) 2. Basics, history 3. DNA sequence assembly and annotation 4. Databases 5. Pair-wise alignment, multiple alignment, BLAST 6. Primer design 7. Phylogeny 8. Metagenomics by 16S rRNA amplicon sequencing 9. Metagenomics by full DNA shotgun sequencing 10. Sequenced based identification and taxonomy 11. Molecular typing of prokaryotes