Protein research has become important in biomedicine, biotechnology, pharmaceutical research and drug design, driven by advances in understanding the human genome and the impact that has. Although there are several types of analytical methods used to investigate protein structure, this volume provides comprehensive coverage of mass spectrometry methods to analyze protein dynamics and protein-protein interactions. Emphasis is placed on providing method details for a very practical step-by-step approach currently not available in one source.
Protein research has become important in biomedicine, biotechnology, pharmaceutical research and drug design, driven by advances in understanding the human genome and the impact that has. Although there are several types of analytical methods used to investigate protein structure, this volume provides comprehensive coverage of mass spectrometry methods to analyze protein dynamics and protein-protein interactions. Emphasis is placed on providing method details for a very practical step-by-step approach currently not available in one source.
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978-0-8138-1793-4 (9780813817934)
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Mark R. Chance, Ph.D., Director of the Case Mass Spectrometry Center at Case Western Reserve University, Cleveland, OH.
Mark R. Chance, Ph.D., Director of the Case Mass Spectrometry Center at Case Western Reserve University, Cleveland, OH.
Section I: Overview of Methodologies for Mass Spectrometry Based Structural Analysis of Proteins.Chapter 1: Overview of Mass Spectrometry Technologies for Examining Protein Structure.Chapter 2: Deuterium Exchange Mass Spectrometry: Principles and Capabilities.Chapter 3: Covalent Labeling Methods for Examining Protein Structure and Protein Interactions.Section 2: Structural Analysis of Protein Complexes.Chapter 4: Deutierium Exchange Approaches for Examining Protein Interactions: Case Studies of Complex Formation.Chapter 5: Transferrin:Receptor Complex Formation Examined by Hdroxyl Radical Footprinting.Chapter 6: Complex Formation in the Actin Cytoskeleton: Deuterium Exchange and Covalent Labeling as Tools to Define Protein Structure and Interactions.Chapter 7: Deuterium Exchange Methods to Examine Virus Assembly.Section 3: Related Approaches in Mass Spectrometry Based Methods of Structural Proteomics.Chapter 8: Computational Approaches to Examining Protein-Protein Interactions: Combining Experimental and Computational Data in the Era of Structural Genomics.Chapter 9: Electrospray Approaches to Examine Intact Proteins and Protein Complexes.Chapter 10: Cross-linking to Examine Protein Complexes.Chapter 11: MS to Examine Protein-Ligand Interactions.Chapter 12: Prospects and Problems on Mass Spectrometry Approaches in Structural Biology
