Methods for Characterizing Receptors.
Radioligand-Binding Methods for Membrane Preparations and Intact Cells
Mary Keen
Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding
Mark E. Olah and Gary L. Stiles
Approaches to the Stable Transfection of G Protein-Coupled Receptors
Andrea Townsend-Nicholson
Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels
Hartmut Lüddens and Esa R. Korpi
The Generation of Receptor-Selective Antibodies
John J. Mackrill
In Situ Hybridization
Judith C.W. Mak and Peter J. Barnes
Immunocytochemical Methods for Investigating Receptor Localization
Maria I. Fonseca and R. Dale Brown
Methods for Studying Receptor Coupling Mechanisms.
Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes
Sebastian Lazareno
Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding
Laura J. Sim, Dana E. Selley, and Steven R. Childers
Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes
Yuji Odagaki and Kjell Fuxe
Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling
Ethan S. Burstein, Tracy A. Spalding, and Mark R. Brann
Identification and Quantitation of G Protein a-Subunits
Ian Mullaney and Graeme Milligan
Covalent Modification of G Proteins by Affinity Labeling
Martin Hohenegger, Michael Freissmuth, and Christian Nanoff
Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection
Stephen R. Ikeda
Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating PhospholipaseC
Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, and Günter Schultz
Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons
Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, and Malcolm P. Caulfield
Methods for Investigating the Regulation of Receptor Function
Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation
Andrew B. Tobin
Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
Alison W. Gagnon and Eamonn Kelly
Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells
Diana M. Slowiejko and Stephen K. Fisher
Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription
Philip A. Iredale, Maura E. Charlton, John D. Alvaro, and Ronald S. Duman
Index.
