This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation
and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these
burgeoning fields.
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
2 colour illustrations, numerous halftones, tables
Maße
Höhe: 241 mm
Breite: 161 mm
Dicke: 22 mm
Gewicht
ISBN-13
978-0-19-963782-9 (9780199637829)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Department of Cell BiologyUniversity of Miami
Department of BiochemistryUniversity of Miami
Preface ; 1. Introduction: The cytoskeleton in the transduction of signal and regulation of cellular function ; 2. Assaying binding and covalent modifications of cytoskeletal proteins ; 3. Methods to study actin assembly and dynamics in yeast ; 4. Signaling complexes: Association of signaling proteins with the cytoskeleton ; 5. Ras-related GTPases and the cytoskeleton ; 6. Applications for analysis of contractile functions in dividing and migrating tissue culture cells ; 7. Video microscopy: Protocols for examining the actin-based motility of Listeria, Shigella, Vaccinia, and Lanthanum-induced endosomes. ; 8. Structural studies on tubulin and microtubules ; 9. Regulation of Intermediate Filament Dynamics ; 10. The study of mRNA-cytoskeleton interactions and mRNA sorting in mammalian cells ; 11. Cell shape control and mechanical signaling through the cytoskeleton ; Appendix ; Index
