This book will help researchers to understand and develop designer antibodies. It provides a practical overview on how to use state-of-the-art technologies in antibody engineering, for the production of recombinant human or mouse monoclonal antibodies. Subjects such as antibody structure relevant to antibody engineering, plant and mammalian expression vectors and hosts, recombinatorial DNA libraries, PCR cloning of single cells, engineering of affinity and biological effector functions, are thoroughly explored by some of the leading experts in each field.
Reihe
Auflage
Sprache
Verlagsort
Zielgruppe
Editions-Typ
Illustrationen
halftones, line figures, tables
Maße
Höhe: 234 mm
Breite: 156 mm
Dicke: 22 mm
Gewicht
ISBN-13
978-0-19-509150-2 (9780195091502)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Professor and Department Chairman, Department of ImmunotechnologyProfessor and Department Chairman, Department of Immunotechnology, Lund University
1: Introduction: Recombinant Antibodies: Future Trends
2: Human Monoclonal Antibodies from V-Gene Repertoiries Expressed on Bacteriophage
3: Synthetic Antibodies
4: Engineering the Antibody Combining Site by Codon-Based Mutagenesis in a Filamentous Phage Display System
5: Human Metaphoric Antibodies from a Phagemid Library
6: Strategies for Humanizing Antibodies
7: Single-Chain Fv Design and Production by Preparative Folding
8: Expressing Antibodies in Escherichia Coli
9: Vector and Approaches for the Eukaryotic Expression of Antibodies and Antibody Fusion Proteins
10: The Cloning of Hybridoma V Regions for their Ectopic Expression in Intracellular and Intercellular Immunisation
11: The Expression of 'Single Chain' Antibodies in Transgenic Plants
