Section I: Introduction and metabolism; Section II: Sampling and extraction; Section III: Physio-chemical methods; Section IV: Pharmacological and immunoassays; Section V: Enzymology and lipid peroxidation; Section VI: Related techniques; Appendix: Eicosanoids: structure, availability, storage and stability; Abbreviations; Introduction to the eicosanoids; Metabolism of prostaglandins and lipoxygenase products: relevance for eicosanoid assay; Tissue sampling and preparation; Extraction of eicosanoids from biological samples; Thin-layer chromatography (including radio thin-layer chromatography and autoradiography of prostaglandins and related compounds; High pressure liquid chromatography in the analysis of arachidonic acid metabolites; Gas chromatography and mass spectrometry of eicosanoids; Measurement of prostaglandins, thromboxanes and leukotrienes by smooth muscle bioassay; Aggregometry techniques for prostanoid study and evaluation; Radioimmunoassay of eicosanoids; Enzyme immunoassay; Cyclo-oxygenases: measurement, purification and properties; Lipoxygenases: measurement, characterization and properties; Lipid peroxidation; Quantitative measurement of arachidonic acid in tissues or fluids; Methods in prostanoid receptor classification; Paf-acether (platelet-activating factor); Appendix; Index
