Part I. Introduction to PCR
A Short History of the Polymerase Chain Reaction
John M. S. Bartlett and David Stirling
PCR Patent Issues
Peter Carroll and David Casimir
Equipping and Establishing a PCR Laboratory
Susan McDonagh
Quality Control in PCR
David Stirling
Part II. Preparation of Nucleic Acid Templates
Extraction of Nucleic Acid Templates
John M. S. Bartlett
Extraction of DNA from Whole Blood
John M. S. Bartlett and Anne White
DNA Extraction from Tissue
Helen Pearson and David Stirling
Extraction of DNA from Microdissected Archival Tissues
James J. Going
RNA Extraction from Blood
Helen Pearson
RNA Extraction from Frozen Tissue
John M. S. Bartlett
RNA Extraction from Tissue Sections
Helen Pearson
Dual DNA/RNA Extraction
David Stirling and John M. S. Bartlett
DNA Extraction from Fungi, Yeast, and Bacteria
David Stirling
Isolation of RNA Viruses from Biological Materials
Susan McDonagh
Extraction of Ancient DNA
Wera M. Schmerer
DNA Extraction from Plasma and Serum
David Stirling
Technical Notes for the Detection of Nucleic Acids
John M. S. Bartlett
Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels
David Stirling
Part III. Basic PCR Methods
PCR Primer Design
David L. Hyndman and Masato Mitsuhashi
Optimization of Polymerase Chain Reactions
Haiying Grunenwald
Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content: Amplification of the Intron 22 Inversion of the FVIII Gene
David Stirling
Rapid Amplification of cDNA Ends
Xin Wang and W. Scott Young III
Randomly Amplified Polymorphic DNA Fingerprinting: The Basics
Ranil S. Dassanayake and Lakshman P. Samaranayake
Microsphere-Based Single Nucleotide Polymorphism Genotyping
Marie A. Iannone, J. David Taylor, Jingwen Chen, May-Sung Li, Fei Ye, and Michael P. Weiner
Ligase Chain Reaction
William H. Benjamin, Jr., Kim R. Smith, and Ken B. Waites
Nested RT-PCR in a Single Closed Tube
Antonio Olmos, Olga Esteban, Edson Bertolini, and Mariano Cambra
Direct PCR from Serum: Application to Viral Genome Detection
Kenji Abe
Long PCR Amplification of Large Fragments of Viral Genomes: A Technical Overview
Raymond Tellier, Jens Bukh, Suzanne U. Emerson, and Robert H. Purcell
Long PCR Methodology
Raymond Tellier, Jens Bukh, Suzanne U. Emerson, and Robert H. Purcell
Part IV. Ultrasensitive and Quantitative PCR
Qualitative and Quantitative PCR: A Technical Overview
David Stirling
Ultrasensitive PCR Detection of Tumor Cells in Myeloma
Friedrich W. Cremer and Marion Moos
Ultrasensitive Quantitative PCR to Detect RNA Viruses
Susan McDonagh
Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics
John M. S. Bartlett
Quantitation of Multiple RNA Species
Ron Kerr
Part V. Transcriptome Analysis
Differential Display: A Technical Overview
John M. S. Bartlett
AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs
Orlando Dominguez, Lidia Sabater, Yaqoub Ashhab, Eva Belloso, and Ricardo Pujol-Borrell
PCR Fluorescence Differential Display
Kostya Khalturin, Sergej Kuznetsov, and Thomas C. G. Bosch
Microarray Analysis Using RNA Arbitrarily Primed PCR
Steven Ringquist, Gaelle Rondeau, Rosa-Ana Risques, Takuya Higashiyama, Yi-Peng Wang, Steffen Porwollik, David Boyle, Michael McClelland, and John Welsh
Oligonucleoti
