Plant Protoplasts and Genetic Engineering IV

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Kiwifruit (Actinidia deliciosa).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Growth of Regenerated Plants.- 4 Variability in Regenerated Plants.- 5 Conclusions and Prospects.- References.- I.2 Regeneration of Plants from Protoplasts of Duboisia.- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Miscellaneous Study.- 7 Conclusion and Prospects.- 8 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Cultivated Strawberry (Fragaria x ananassa) and Wild Strawberry (Fragaria vesca).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Genetic Transformation in Strawberry.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Sweet Potato (Ipomoea batatas L. Lam.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Characterization of Protoplast-Derived Plants.- 6 Fusion of Protoplasts.- 7 Summary and Conclusion.- 8 Protocol.- References.- I.5 Regeneration of Plants from Alfalfa (Medicago sativa L.) Protoplasts by Direct Embryogenesis.- 1 Introduction.- 2 Procedures.- 3 Results.- 4 Discussion.- 5 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Adsuki Bean (Phaseolus angularis Wight).- 1 Introduction.- 2 Material and Methods.- 3 Results and Discussion.- 4 Summary.- References.- I.7 Regeneration of Plants from Protoplasts of Some Stone Fruits (Prunus spp.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Protoplast Fusion and Somatic Hybridization Studies with Prunus Genotypes.- 6 Miscellaneous Studies with Prunus Protoplasts.- 7 Conclusions.- 8 References.- I.8 Regeneration of Plants from Protoplasts of Winged Bean (Psophocarpus tetragonolobus L. DC.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Protoplast Purification.- 4 Protoplast Culture.- 5 Plant Regeneration from Protoclones.- 6 Conclusion.- 7 Protocol.- References.- I.9 Regeneration of Plants from Protoplasts of Eggplant (Solanum melongena L.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Fusion of Protoplasts.- 6 Summary and Conclusion.- 7 Protocol.- References.- I.10 Regeneration of Plants from Protoplasts of Sorghum (Sorghum vulgare).- 1 Introduction.- 2 Embryogénie Callus Formation and Establishment of Suspension Culture.- 3 Protoplast Isolation and Culture.- 4 Plant Regeneration.- 5 Protoplast Transformation by PEG-Mediated DNA Transfer.- 6 Conclusion.- 7 Protocol.- References.- Section II Genetic Transformation.- II. 1 Transformation in Horseradish (Armoracia rusticana): Hairy Roots Incited by Agrobacterium rhizogenes Infection.- 1 Introduction.- 2 Varieties of Horseradish.- 3 Induction of Hairy Roots.- 3 Confirmation of Ri Plasmid-Direction Transformation.- 5 Growth of Hairy Roots.- 6 Bioreactor for Hairy Root Culture.- 7 Peroxidase Productivity of Hairy Roots.- 8 Regeneration of Hairy Roots.- 9 Summary and Conclusion.- References.- II.2 Transformation in Sugar Beet (Beta vulgaris L.).- 1 Introduction.- 2 Transformation by Direct Gene Transfer.- 3 Transformation by Agrobacterium tumefaciens.- 4 Conclusions.- References.- II.3 Transformation in Oilseed Rape (Brassica napus L.).- 1 Introduction.- 2 Susceptibility of Brassica napus to Agrobacterium tumefaciens Infection.- 3 Transformation of Tissue Explants.- 4 Transformation of Protoplasts.- 5 Gene Transfer and Regeneration Protocol.- 6 Summary and Conclusion.- 7 Culture Media and Solutions.- References.- II.4 Transformation in Chickpea (Cicer arietinum L.).- 1 Introduction.- 2 Agrobacterium Chickpea Interaction.- 3 Analysis of Transformed Tissues.- 4 Summary and Conclusions.- References.- II.5 Transformation in Citrus.- 1 Introduction.- 2 Review of Previous Research on Citrus Transformation.- 3 Transformation Parameters.- 4 Evaluation of Transformation.- 5 Conclusions and Prospects.- 6 Protocol.- References.- II.6 Transformation in Muskmelon (Cucumis melo L.).- 1 Introduction.- 2 A. tumefaciens-Mediated Transformation and Regeneration of Muskmelon Plants.- 3 Summary and Conclusions.- References.- 11.7 Transformation in Cucumber (Cucumis sativus L.).- 1 Introduction.- 2 Materials and Methods.- 3 Experimental Results.- 4 Preliminary Field Testing of Plants Which Express the CMV-C Coat Protein Gene.- 5 Second Field Testing of Transgenic Cucumber Plants That Express the CMV Coat Protein Gene.- 6 Conclusion.- References.- II.8 Transformation in Soybean (Glycine max L.).- 1 Introduction.- 2 Delivery of DNA into Soybean Cells.- 3 Regeneration of Transformed Soybeans.- 4 Conclusions and Future Prospects.- References.- II.9 Transformation of Lettuce (Lactuca sativa L.).- 1 General Account.- 2 Transformation in Lactuca sativa L.- 3 Inoculation of Agrobacterium and Selection of Transformants.- 4 GUS Assay and Kanamycin Resistance in the Self-Pollinated Progeny.- 5 Histochemical Localization of Salicylic Acid and Bacterial Stress-Induced GUS Activity.- 6 Southern Blot Analysis.- 7 Conclusions and Prospects.- 8 Protocol.- References.- II. 10 Transformation of Birds-Foot Trefoil (Lotus corniculatus).- 1 Introduction.- 2 Transformation of Lotus corniculatus.- 3 An Overview of Genetic Transformation of Forage Legumes.- 4 Concluding Remarks.- 5 Protocol.- References.- II.11 Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection.- 1 Introduction.- 2 Review of Transformation Work in Tomato.- 3 Intranuclear Microinjection for Transformation of Tomato Callus Cells.- 4 Transformation of Tomato with Mutagenized Coat Protein (MCP) Gene.- 5 Summary and Conclusion.- References.- II.12 Transformation in Cassava (Manihot esculenta Crantz).- 1 Introduction.- 2 Experiments with a Particle Gun: Transient Expression of the GUS Gene.- 3 Transformation of Leaf Disks with Agrobacterium tumefaciens.- 4 Transformation of Leaf Lobes by Bombardment with an Agrobacterium tumefaciens Suspension.- 5 Transformation of Somatic Embryos with Agrobacterium tumefaciens.- 6 Antibiotics and Selection of Transformed Tissues.- 7 Summary and Conclusions.- References.- II.13 Transformation of Tobacco (Nicotiana clevelandii and N. benthamiana).- 1 Introduction.- 2 Transformation Studies on Nicotiana.- 3 Transformation of Nicotiana clevelandii and N. benthamiana.- 4 Prospects and Conclusions.- References.- II.14 Transformation of Petunia and Corn Plants (Petunia hybrida and Zea mays) Using Agrobacterium tumefaciens and the Shoot Ape.- 1 Introduction.- 2 Methods.- 3 Results.- 4 Discussion.- 5 Assumptions.- 6 Conclusions.- References.- II.15 Transformation in Spruce (Picea Species).- 1 Introduction.- 2 Gene Transfer Systems.- 3 Selection.- 4 Gene Expression.- 5 Regeneration of Transformed White Spruce.- 6 Summary.- References.- 11.16 Transformation of Pea (Pisum sativum L.).- 1 Introduction.- 2 Pea Tissue Culture/Regeneration in Vitro.- 3 Transformation.- 4 Concluding Remarks.- References.- II.17 Transformation of Sugarcane.- 1 Why Genetic Engineering Is Attractive for Sugarcane Variety Improvement.- 2 Sugarcane Tissue Culture.- 3 Gene Transfer Techniques.- 4 Promoters and Marker Genes for Sugarcane Transformation.- 5 Prospects.- References.- II.18 Transformation of Stylosanthes Species.- 1 Introduction.- 2 Tissue and Protoplast Culture of Stylosanthes spp.- 3 Agrobacterium-Mediated Transformation.- 4 Transient Gene Expression in S. humilis Using DNA-Coated Microprojectiles.- 5 Selection of Transformed Tissues.- 6 Reporter Gene Expression in Stably Transformed Tissues.- 7 Genes for Improvement.- 8 Summary.- References.- II.19 Transformation in Grapevine (Vitis spp.).- 1 Introduction.- 2 Genetic Transformation of Grape.- 3 Conclusions and Future Prospects.- References.