Part 1 Why use histochemistry for a better understanding of pharmacology and toxicology?, Richard W.Horobin: what is histochemistry?; information generated by histochemical methods; a look at the "what", "how much" and "how many" questions; how and why to do histochemistry; selection of procedures; how to assess the significance of staining; using histochemistry in pharmacology and toxicology. Part 2 Whole-body autoradiography of pharmaceuticals and chemicals, P.Bernard: theoretical aspects; whole-body autoradiography. Part 3 Image analysis, fluorescence and laser microscopy, J.S.Ploem: fluorescence image analysis; laser scanning microscopy; light and scanning electron microscopy combined in one instrument. Part 4 The measurement of free calcium to assess cellular function and injury using digital imaging fluorescence microscopy, B.F.Trump et al: methodology; examples of DIFM applications; potential future applications for DIFM in toxicologic pathology. Part 5 Flow cytometry to characteristic normal and abnormal cell function, M.G.Ormerod and J.A.Green: measurement of DNA and the cell cycle; measurement of cellular antigens; combined measurement of protein and DNA; drug uptake and reaction; intracellular enzymes; measurement of the integrity of cells; other intracellular probes; chromosome analysis. Part 6 Cytochemical quantification of enzymes in frozen tissue sections, C.J.F.van Noorden and R.Gossrau: ultra-microchemistry; cytophotometry; applications. Part 7 Fixation of tissue for histochemistry, D.Hopwood: techniques for fixation; fixatives; the biochemical nature of the material to be fixed; other factors affecting fixation; specific problems; miscellaneous problems; constraints and hazards. Part 8 Immunocytochemical methods to assess cellular malfunction, J.E.Beesley: immunocytochemical techniques - practical considerations, use in toxicology and pathology. Part 9 Cell markers and processes related to chemically induced carcinogenesis, P.Bannasch et al: localizable macromolecules; enzyme histochemistry and immunohistochemistry; biochemical microanalysis; "in situ" hybridization. Part 10 The mechanistic basis of chemical and drug-induced nephrotoxicity, W.J.Gregg et al: the biochemical basis for renal target-selective injury; glomerular injury; proximal tubular injury; renal medulla; future trends in the use of histochemical and immunohistochemical techniques. Part 11 Pollutant-induced toxity in the marine environment, M.W.Moore: approach; cellular reactions.
