Ultra-High Performance Liquid Chromatography and Its Applications

Explores both the benefits and limitations of new UHPLCtechnology
High performance liquid chromatography (HPLC) has been widelyused in analytical chemistry and biochemistry to separate,identify, and quantify compounds for decades. The science of liquidchromatography, however, was revolutionized a few years ago withthe advent of ultra-high performance liquid chromatography (UHPLC),which made it possible for researchers to analyze sample compoundswith greater speed, resolution, and sensitivity.
Ultra-High Performance Liquid Chromatography and ItsApplications enables readers to maximize the performance ofUHPLC as well as develop UHPLC methods tailored to their particularresearch needs. Readers familiar with HPLC methods will learn howto transfer these methods to a UHPLC platform and vice versa. Inaddition, the book explores a variety of UHPLC applicationsdesigned to support research in such fields as pharmaceuticals,food safety, clinical medicine, and environmental science.
The book begins with discussions of UHPLC method development andmethod transfer between HPLC and UHPLC platforms. It then examinespractical aspects of UHPLC. Next, the book covers:
* Coupling UHPLC with mass spectrometry
* Potential of shell particles in fast liquid chromatography
* Determination of abused drugs in human biological matrices
* Analyses of isoflavones and flavonoids
* Therapeutic protein characterization
* Analysis of illicit drugs
The final chapter of the book explores the use of UHPLC in drugmetabolism and pharmacokinetics studies for traditional Chinesemedicine.
With its frank discussions of UHPLC's benefits and limitations,Ultra-High Performance Liquid Chromatography and ItsApplications equips analytical scientists with the skills andknowledge needed to take full advantage of this new separationtechnology.