Modern Liquid Chromatography of Macromolecules

Name: Modern Liquid Chromatography of Macromolecules
Brand: Elsevier
Price: 245.0 EUR
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L. Z. Vilenchik(Autor*in)

B. G. Belenkii(Herausgeber*in)

Elsevier (Verlag)

Erschienen am 14. Mai 2014

455 Seiten

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Front Cover
Modern Liquid Chromatography of Macromolecules
Copyright Page
Contents
Journal of Chromatography Library
Foreword
Preface
Chapter 1. General theory of chromatography
1.1. Characterization of the chromatographic method
1.2. Methods of the description of the chromatographic process for some specific chromatographic systems
1.3. Kinetics of the chromatographic process
1.4. Method of statistical moments in chromatography
1.5. Analysis of chromatographic spreading
1.6. Optimization of the chromatographic process
References
Chapter 2. Main features of the chromatography of macromolecules
2.1. Change in the free energy of the macromolecules during interphase transitions as the main characteristic of the behaviour of the substance in the chromatographic process
2.2. Essential features of the change in the free energy of macromolecules during interphase transitions. Two main variations of the chromatography of polymers
2.3. Gel-permeation chromatography of polymers (GPC) on rigid sorbents
2.4. Main relationships of adsorption chromatography of polymers on rigid macroporous sorbents
2.5. Main features of the chromatography of flexible-chain polymers on swelling macroporous sorbents
References
Chapter 3. Interpretation of data on the analysis of polymers by gel chromatography
3.1. Aims of interpretation
3.2. Levels of interpretation
3.3. Calibration dependences in GPC
3.4. Calibration of the chromatograph
3.5. Determination of calibration dependence in the absence of polymer standards
3.6. Determination of constants K. and a in the Mark-Kuhn-Houwink equation from experimental GPC data
3.7. Determination of molecular-weight characteristics of polymers without correcting chromatographic data for 'instrumental spreading' (first level of interpretation)
3.8. Determination of molecular weight characteristics of polymers in which 'instrumental spreading' described by a Gaussian function is taken into account (second level of interpretation)
3.9. Determination of molecular-weight characteristics of polymers in which the asymmetry of the chromatograms of homopolymers is taken into account (third level of interpretation)
3.10. Use of asymmetrical functions for the description of 'instrumental spreading' (fourth level of interpretation)ss
3.11 . Error in the determination of molecular weights by the GPC method
References
Chapter 4. Methodological problems of gel-permeation chromatography (GPC)
4.1. Sorbents used in the GPC of polymers
4.2. Preparation of chromatographic columns
4.3. Narrow-disperse polymer standards for column calibration in the GPC of polymers
4.4. Solvents for the GPC of polymers
4.5. Detectors
4.6. Equipment for liquid chromatography
4.7. Procedures of the GPC of polymers
4.8. Preparative GPC
4.9. Use of recycling for increasing the efficiency of the chromatographic system
4.10. Vacancy GPC
4.11. High-speed GPC
4.12. Microcolumn GPC
References
Chapter 5. Use of gel-permeation chromatography for the analysis of macro- molecules investigation of their interaction and the characterization of the porous structure of sorbentsss
5.1. Gel-permeation chromatography of oligomers
5.2. Gel-permeation chromatography of polyelectrolytes
5.3. Analysis of branched-chain polymers by a combination of GPC and viscometry
5.4. Determination of the MWD of branched polymers by a combination of GPC and sedimentation
5.5. Combination of GPC with independent methods for obtaining average mole- cular characteristics
s5.6. Determination of the MWD of random copolymers by combining GPC, viscometry, osmometry, light scattering and pyrolysis gas chromatography
5.7. Determination of the composition and the MWD of block copolymers
5.8. Combined chromatographic methods for the analysis of complex polymer systems by gel-permeation, thin-layer and pyrolysis gas chromatography
5.9. Use of GPC for investigation of isomerization, association and complexation of proteins and determination of their molecular weight
5.10. Chromatographic porosimetry
References
Chapter 6. High-performance gel-filtration chromatography (HPGFC)
6.1. Sorbents for HPGFC
6.2. Applications of HPGFC
References
Chapter 7. Thin-layer chromatography of polymers
7.1. Development of thin-layer chromatography of polymers and its variations
7.2. Adsorption TLC of polymers
7.3. Precipitation and extraction TLC of polymers
7.4. Dependence of spot shape on concentration in the TLC of polymers
7.5. Investigations of the microstructure of polymers by TLC
7.6. TLC of block and graft copolymers
7.7. TLC of oligomers
7.8. Some methodological problems of the TLC of polymers
References
Chapter 8. One-phase chromatography: field-flow fractionation and hydrodynamic chromatography
8.1. Theoretical basis of FFF
8.2. Types of FFF
8.3. Separation efficiency and speed in FFF
8.4. FFF programming
8.5. Comparison of FFF with exclusion chromatography and other chromatographic methods of fractionation of macromolecules and microparticles
8.6. Polymer fractionation by FFF
8.7. Future prospects of using various types of FFF
8.8. Hydrodynamic chromatography
References
Subject Index
Atomic Absorption Spectrometry
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