Chromatographic & Electrophoretic Techniques, Fourth Edition, Volume II: Zone Electrophoresis presents the methods of zone electrophoresis. This book discusses all the techniques that can be applied routinely.
Organized into 17 chapters, this volume begins with an overview of the fundamental principle of zone electrophoresis. This text then examines the apparatus in common use for paper electrophoresis and discusses paper electrophoresis at low voltages. Other chapters describe the apparatus that combines several advantages resulting from high voltage paper electrophoresis and thin-layer chromatography. This book discusses as well the advantages of cellulose acetate membrane filter paper as a supporting medium for zone electrophoresis. The final chapter deals with preparative acrylamide gel electrophoresis on a small scale, which may involve either continuous electrophoretic elution or extraction of the RNA from zones cut out of the gel.
This book is a valuable resource for biochemists, clinical chemists, clinicians, technicians, biophysicists, pathologists, and medical researchers.
Auflage
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-1-4832-7991-6 (9781483279916)
Schweitzer Klassifikation
Editor and AuthorsPreface1. General Principles of Zone Electrophoresis2. Paper Electrophoresis at Low Voltage3. High Voltage Paper Electrophoresis4 . Thin Layer Electrophoresis5. Cellulose Acetate Electrophoresis and Immuno-Diffusion Techniques6. Agar Gel Electrophoresis and Immuno-Electrophoresis7. Starch Gel Electrophoresis8. Separation and Visualization of Enzymes on Gels9. Acrylamide Gel Disc Electrophoresis Techniques of Disc Electrophoresis Protein Patterns in Human Serum; Interpretation10. Concentration Gradient Polyacrylamide Gel Electrophoresis11. Isoelectric Focusing of Proteins12. Electrophoresis of Proteins on SDS Polyacrylamide Gels13. Quantitative Immunoelectrophoresis14. Preparative Block Electrophoresis15. Preparative Acrylamide Disc Electrophoresis Section 1 Section 216. Models for Macromolecules, Nucleic Acids and Proteins17. Electrophoresis of RNA in Polyacrylamide and Agarose GelsIndex
