Chromatographic and Electrophoretic Techniques, Volume II: Zone Electrophoresis, Second Edition presents tried and tested methods of zone electrophoresis, which has been carried out on commercially available apparatus offering many advantages to the majority of laboratories.
This book is divided into 14 chapters and begins with a brief introduction to the general principles of zone electrophoresis. The subsequent chapters deal with the principles, instrumentation, and applications of various methods of zone electrophoresis, including low- and high-voltage paper, cellulose-acetate, thin-layer, agar- and starch-gel, and preparative block electrophoresis. The remaining chapters cover other zone electrophoresis methods, such as acrylamide gel, disc, acrylamide flat gel, and continuous electrophoresis.
This book is of value to analytical chemists and biologists.
Auflage
Sprache
Verlagsort
Saint Louis
Großbritannien
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-1-4832-2209-7 (9781483222097)
Schweitzer Klassifikation
Editors and AuthorsPreface to Second EditionPreface1. General Principles of zone Electrophoresis2. Paper Electrophoresis at Low Voltage Techniques of Paper Electrophoresis The Separation of Serum Proteins, Lipoproteins, Glycoproteins, Phosphate, Cholesterol Clinical Interpretations of Serum Electrophoretic Patterns Hemoglobins Carbohydrate-Protein Compounds and Acid Glycosaminoglycans One Way Separation of Aminoacids Two Way Screening Procedures for Aminoacids, Indoles and Imidazoles3. Cellulose Acetate Electrophoresis and Immunodiffusion Techniques4. Thin-Layer Electrophoresis5. High Voltage Paper Electrophoresis6. Agar Gel Electrophoresis and Immuno-Electrophoresis7. Preparative Block Electrophoresis8. Starch Gel Electrophoresis Techniques of Starch Gel Electrophoresis Micro Starch Gel Electrophoresis Application to the Study of Serum and Urine Proteins Hemoglobins9. Starch Gel Zone Electrophoresis of Enzymes10. Disc Electrophoresis on Acrylamide Gels Techniques of Disc Electrophoresis Protein Patterns in Human Serum; Interpretation Separation of Milk Proteins C.S.F. Electrophoresis11. Disc Electrophoresis of RNA in Polyacrylamide Gels Electrophoresis of RNA The Fractionation of High Molecular Weight RNA12. Acrylamide Flat Slab Gel Electrophoresis Horizontal Gel Slab Electrophoresis The Relationship between Mobility, Gel Concentration and Molecular Weight Micro Gel Electrophoresis Vertical Flat Bed Gel Electrophoresis13. Preparative Acrylamide Disc Electrophoresis Section 1. Section 2.14. Continuous ElectrophoresisIndex