Chromatography Today provides a comprehensive coverage of various separation methods: gas, liquid, thin-layer, and supercritical fluid-chromatography, and capillary electrophoresis. Particular attention is paid to the optimization of these techniques in terms of kinetic parameters and retention mechanisms. When these facts are understood, method selection and optimization becomes a more logical process. Sample preparation methods are treated fully as they frequently represent an integral part of the total analytical method. Also described are preparative-scale separations used for isolating significant amounts of product which are generally achieved under conditions that are not identical to those used for analytical separations. The most common hyphenated methods used for sample identification are discussed from the perspective of the information they yield and the requirements of common interfaces.
The scope and level of discussion are designed to be appropriate for various user groups. This book should be suitable for use as a graduate-level student textbook in separation science, a text for professional institutes offering short courses in chromatography, and as a self-study guide for chromatographers to refresh their knowledge of the latest developments in the field. The book is extensively illustrated with over 200 figures, 110 tables and 3,300 references, largely to the contemporary literature.
Auflage
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-0-444-59619-2 (9780444596192)
Schweitzer Klassifikation
1. Fundamental Relationships of ChromatographyIntroduction. Retention. Flow in Porous Media. Band BroadeningMechanism. Peak Shape Models. Parameters Affecting Resolution.Optimization of Kinetic Column Variables. Principles ofQuantitation in Column Chromatography. References. 2. TheColumn in Gas Chromatography Introduction. Packed ColumnGas-Liquid Chromatography. Open Tubular Columns. Evaluation ofthe Quality of Open Tubular Columns. Retention in Gas-LiquidChromatography. Gas-Solid Chromatography. Preparative-Scale GasChromatography. References. 3. Instrumental Aspects of GasChromatography Introduction. Pneumatic Systems. ThermalZones. Sample Inlets. Detection Devices for Gas Chromatography.Column Connectors and Effluent Splitters. References. 4 TheColumn in Liquid Chromatography Introduction. Column PackingMaterials. Column Preparation. Column Evaluation and TestMethods. Retention Mechanisms in Liquid Chromatography. MethodDevelopment Strategies for Liquid Chromatography. Preparative-Scale Liquid Chromatography. Capillary Electrophoretic SeparationMethods. References. 5. Instrumental Aspects of High PressureLiquid Chromatography Introduction. Requirements with Respectto Extracolumn Dispersion. Solvent Reservoirs and SolventDegassing. Solvent Delivery Systems. Injection Devices.Detectors. References. 6. Supercritical FluidChromatography Introduction. Column Selection. Mobile PhaseSelection. Parameters Affecting Retention. Instrumental Aspectsof Supercritical Fluid Chromatography. References. 7. Thin-Layer Chromatography Introduction. TheoreticalConsiderations. Stationary Phases for TLC. DevelopmentTechniques. Mobile Phase Optimization. Quantitative Evaluationof Thin-Layer Chromatography. References. 8. SamplePreparation for Chromatographic Analysis Introduction.Problems in Obtaining a Representative Sample. Isolation andConcentration Techniques Using Physical Methods. Isolation andConcentration Techniques Using Solvent Extraction. Sample CleanupUsing Liquid Chromatography. Sorption Techniques for TraceEnrichment of Organics in Water. Multidimensional and MultimodalSample Fractionation and Trace Enrichment Techniques. HeadspaceSampling. Air Sampling. Derivatization Techniques for GasChromatography. Derivatization Techniques for LiquidChromatography. Derivatives for the Chromatographic Separationof Inorganic Anions and Cations. Visualization Techniques forThin-Layer Chromatography. Qualitative Identification andMicroreaction Techniques for Gas Chromatography. Separation ofStereoisomers. Pyrolysis Gas Chromatography. References. 9.Hyphenated Methods for Identification after ChromatographicSeparation Introduction. Instrumental Requirements for MassSpectrometry. Interface Requirements for Mass Spectrometry.Interface Requirements for Chromatographic Sample IntroductionSystems in Mass Spectrometry. Data Management and QuantitativeIon Monitoring in Mass Spectrometry. Instrumental Requirementsfor Chromatographic Sample Introduction Systems in FourierTransform Infrared Spectroscopy. Liquid Chromatography/NuclearMagnetic Resonance Spectroscopy. References. Subject Index.
