Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on cells and tissues. The selection first offers information on phase contrast and interference microscopy in cytology and birefringence and dichroism of cells and tissues. Topics include observational methods and results, quantitative techniques, nerve and muscle fibers, chloroplasts, cytoplasm, and nucleus. The book then examines X-ray microscopy and X-ray absorption analysis, including theoretical considerations of X-ray microscopy of biological materials and absorption and production of X-rays. The publication ponders on microtomy and manometric techniques for single cells and electron microscopy of microorganisms. Discussions focus on Cartesian diver respirometers, capillary respirometers, preliminary preparation of materials for drying, and techniques for studying dried specimens. The selection is highly recommended for students and readers interested in the physical techniques used in biological research.
Auflage
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-1-4832-7077-7 (9781483270777)
Schweitzer Klassifikation
ContentsList of Contributors vPreface to First Edition of Volume IIIPreface to Second EditionChapter 1 Phase Contrast and Interference Microscopy in Cytology I. Introduction II. Observational Methods and Results III. Quantitative Techniques References Chapter 2 Birefringence and Dichroism of Cells and Tissue I. Introduction II. Plant Cell Walls III. Cytoplasm IV. Chloroplasts V. Nerve Fibers VI. Muscle Fibers VII. Nucleus References Chapter 3 X-Ray Microscopy and X-Ray Absorption Analysis I. Introduction II. Production of X-Rays III. Absorption of X-Rays IV. The Formation of X-Ray Images V. Theoretical Considerations of X-Ray Microscopy of Biological Materials VI. Equipment VII. Preparation of the Biological Material for Historadiography and a Discussion of Errors VIII. Some Applications of Microradiography References Chapter 4 Microtomy I. Introduction II. The Specimen and Embedding III. Microtomes IV. Knives and Knife Sharpening V. Measurement of Section Thickness as a Criterion of Over-all Performance VI. Resolution and Section Thickness References Chapter 5 Manometric Techniques for Single Cells I. Introduction II. Capillary Respirometers III. Cartesian Diver Respirometers References Chapter 6 Electron Microscopy of Microorganisms I. Introduction II. Preliminary Preparation of Materials for Drying III. Techniques for Drying Specimens IV. Techniques for Studying Dried Specimens V. Conclusions References Author indexSubject Index
