1 - Front Cover [Seite 1]
2 - Instrumental Liquid Chromatography: A Practical Manual on High-Performance Liquid Chromatographic Methods [Seite 4]
3 - Copyright Page [Seite 5]
4 - Contents [Seite 6]
5 - Journal of Chromatography Library [Seite 10]
6 - Preface to the First Edition [Seite 12]
7 - Preface to the Second Edition [Seite 14]
8 - PART 1: FUNDAMENTALS AND INSTRUMENTATION [Seite 16]
8.1 - Chapter 1. Introduction and historical background [Seite 18]
8.1.1 - References [Seite 21]
8.2 - Chapter 2. Basic principles and terminology [Seite 23]
8.2.1 - General resolution equation [Seite 32]
8.2.2 - Calculation of optimum column length [Seite 32]
8.2.3 - References [Seite 36]
8.3 - Chapter 3. The chromatographic support and column [Seite 38]
8.3.1 - Introduction [Seite 38]
8.3.2 - Sources of band broadening [Seite 39]
8.3.3 - Role of particle size in LC columns [Seite 44]
8.3.4 - Porous layer supports [Seite 49]
8.3.5 - Totally porous (microparticulate) supports [Seite 50]
8.3.6 - Dependence of column efficiency on operating conditions [Seite 52]
8.3.7 - Columns for high-pressure LC [Seite 54]
8.3.8 - Column efficiency and internal diameter [Seite 55]
8.3.9 - Methods of packing chromatographic columns [Seite 58]
8.3.10 - Microbore columns [Seite 67]
8.3.11 - References [Seite 69]
8.4 - Chapter 4. Liquid chromatographic instrumentation [Seite 72]
8.4.1 - Introduction [Seite 72]
8.4.2 - Tubing and tube fittings [Seite 73]
8.4.3 - Safety considerations [Seite 74]
8.4.4 - Solvent delivery systems [Seite 76]
8.4.5 - Gradient elution devices [Seite 85]
8.4.6 - Other components of the solvent delivery system [Seite 90]
8.4.7 - Sample introduction [Seite 95]
8.4.8 - Chromatographic column and couplings [Seite 103]
8.4.9 - Detectors [Seite 108]
8.4.10 - Fraction collectors [Seite 110]
8.4.11 - Measurement of mobile phase flow-rate [Seite 111]
8.4.12 - Presentation of results [Seite 113]
8.4.13 - Availability of LC equipment [Seite 113]
8.4.14 - References [Seite 114]
8.5 - Chapter 5. Liquid chromatographic detection systems [Seite 116]
8.5.1 - Introduction [Seite 116]
8.5.2 - Principal requirements of an LC detector [Seite 119]
8.5.3 - Photometric detectors [Seite 119]
8.5.4 - Fluorescence detection [Seite 125]
8.5.5 - Refractive index detectors [Seite 128]
8.5.6 - Phase transformation detectors [Seite 132]
8.5.7 - Other detection devices [Seite 133]
8.5.8 - Final comment on instrument design [Seite 136]
8.5.9 - References [Seite 137]
8.6 - Chapter 6. Modern electronic technology and its impact on LC automation [Seite 140]
8.6.1 - Introduction [Seite 140]
8.6.2 - Function of electronics in LC instrumentation [Seite 140]
8.6.3 - Selection and optimisation of separation conditions [Seite 141]
8.6.4 - Control of the separation conditions [Seite 142]
8.6.5 - Unattended operation [Seite 142]
8.6.6 - Special detection techniques [Seite 143]
8.6.7 - Calculation of column performance parameters [Seite 146]
8.6.8 - References [Seite 146]
9 - PART 2: FACTORS INFLUENCING CHROMATOGRAPHIC SELECTIVITY [Seite 148]
9.1 - Chapter 7. Nature of the mobile phase [Seite 150]
9.1.1 - Introduction [Seite 150]
9.1.2 - Methods of separation in the liquid phase [Seite 151]
9.1.3 - Classification of mobile phases [Seite 154]
9.1.4 - Development of chromatographic methods [Seite 160]
9.1.5 - Elution behaviour of complex mixtures of dissimilar compounds [Seite 169]
9.1.6 - References [Seite 189]
9.2 - Chapter 8. Liquid-solid (adsorption) chromatography [Seite 190]
9.2.1 - Introduction [Seite 190]
9.2.2 - Range of sample applicability [Seite 190]
9.2.3 - Types of adsorptive packings [Seite 193]
9.2.4 - Mechanism of adsorption chromatography [Seite 196]
9.2.5 - Choice of separating conditions [Seite 199]
9.2.6 - Practical aspects of adsorption chromatography [Seite 200]
9.2.7 - References [Seite 207]
9.3 - Chapter 9. Liquid-iquid (partition) chromatography [Seite 208]
9.3.1 - Introduction [Seite 208]
9.3.2 - Range of sample applicability [Seite 209]
9.3.3 - General considerations [Seite 210]
9.3.4 - Types of liquid-liquid phase systems [Seite 211]
9.3.5 - References [Seite 217]
9.4 - Chapter 10. Bonded-phase chromatography [Seite 218]
9.4.1 - Introduction [Seite 218]
9.4.2 - Range of sample applicability [Seite 218]
9.4.3 - Classification of bonded-phase packings [Seite 219]
9.4.4 - Selection of column packings and solvent to use as mobile phase [Seite 223]
9.4.5 - References [Seite 234]
9.5 - Chapter 11. Ion-exchange and ion-pair chromatography [Seite 236]
9.5.1 - Part I: Ion-exchange chromatography [Seite 236]
9.5.1.1 - Introduction [Seite 236]
9.5.1.2 - Range of sample applicability [Seite 237]
9.5.1.3 - Mechanism of ion-exchange separations [Seite 243]
9.5.1.4 - Structure of column packings for ion-exchange chromatography [Seite 246]
9.5.1.5 - Commercially available ion-exchange materials [Seite 250]
9.5.1.6 - Practical aspects of ion-exchange chromatography [Seite 253]
9.5.2 - Part II: Ion-pair partition chromatography [Seite 259]
9.5.2.1 - Range of sample applicability [Seite 260]
9.5.2.2 - Choice of column packing for ion-pair chromatography [Seite 261]
9.5.2.3 - Factors influencing selection of mobile phase [Seite 261]
9.5.2.4 - "Ion-pair" chromatography of basic substances [Seite 263]
9.5.2.5 - Ion chromatography [Seite 264]
9.5.2.6 - References [Seite 265]
9.6 - Chapter 12. Steric exclusion chromatography [Seite 268]
9.6.1 - Introduction [Seite 268]
9.6.2 - Range of applicability of the method [Seite 268]
9.6.3 - Mechanism of separation [Seite 269]
9.6.4 - Column packings for steric exclusion chromatography [Seite 272]
9.6.5 - Choice of mobile phases for steric exclusion chromatography [Seite 281]
9.6.6 - General scope of steric exclusion chromatography [Seite 283]
9.6.7 - References [Seite 298]
10 - PART 3: USES OF LIQUID CHROMATOGRAPHIC PROCEDURES [Seite 300]
10.1 - Chapter 13. Qualitative analysis [Seite 302]
10.1.1 - Introduction [Seite 302]
10.1.2 - Methods of establishing or confirming the identity of an eluting peak [Seite 303]
10.1.3 - Other considerations when seeking to identify an eluted component [Seite 310]
10.1.4 - References [Seite 311]
10.2 - Chapter 14. Quantitative analysis [Seite 312]
10.2.1 - Introduction [Seite 312]
10.2.2 - Sources of error in chromatographic analysis [Seite 313]
10.2.3 - Manual methods of integration made after completion of the analysis [Seite 324]
10.2.4 - Integration made during the course of the analysis [Seite 326]
10.2.5 - Normalisation of the peaks [Seite 328]
10.2.6 - Normalisation of peaks with correction factors [Seite 329]
10.2.7 - Calibration by means of an external standard [Seite 329]
10.2.8 - Calibration using an internal standard [Seite 330]
10.2.9 - References [Seite 331]
10.3 - Chapter 15. Practical aspects of trace analysis [Seite 332]
10.3.1 - Introduction [Seite 332]
10.3.2 - Sample pretreatment [Seite 332]
10.3.3 - Sample injection [Seite 337]
10.3.4 - Chromatographic considerations [Seite 339]
10.3.5 - Detection considerations [Seite 344]
10.3.6 - Quantitation of minor components [Seite 348]
10.3.7 - References [Seite 348]
10.4 - Chapter 16. Practical aspects of preparative liquid chromatography [Seite 350]
10.4.1 - Introduction [Seite 350]
10.4.2 - Available methods for increasing the sample throughput of chromatographic columns [Seite 351]
10.4.3 - Effect of column geometry o n chromatographic resolution [Seite 352]
10.4.4 - Considerations on the chromatographic support [Seite 354]
10.4.5 - Practical aspects of preparative liquid chromatography [Seite 356]
10.4.6 - Applications of preparative chromatography [Seite 361]
10.4.7 - Industrial-scale chromatographic separations [Seite 364]
10.4.8 - References [Seite 365]
11 - PART 1: APPLICATIONS OF LIQUID CHROMATOGRAPHY [Seite 366]
11.1 - Chapter 17. Published LC applications [Seite 368]
11.1.1 - Pharmaceutical analysis [Seite 369]
11.1.2 - Therapeutic drug monitoring of body fluids [Seite 372]
11.1.3 - Biochemical analysis [Seite 373]
11.1.4 - Food analysis [Seite 376]
11.1.5 - Agricultural chemicals and plant growth regulators [Seite 379]
11.1.6 - Oil and petroleum analysis [Seite 380]
11.1.7 - Petrochemical and related compounds [Seite 381]
11.1.8 - Inorganic and organometallic compounds [Seite 383]
11.1.9 - Polymer analysis (incl . additives) [Seite 384]
11.2 - Appendix 1. International system of units (SI) [Seite 386]
11.3 - Appendix 2. Derivation of the general resolution equation [Seite 387]
11.4 - Appendix 3. Comparison of the U.S. (A.S.T.M.) and B.S.S. sieve sizes in relation to aperture size in micrometers [Seite 389]
11.5 - Appendix 4. Suppliers of liquid chromatographic instrumentation and components [Seite 390]
11.6 - Appendix 5. Solvent selection for infrared detectors [Seite 396]
11.7 - Appendix 6. Standard practice for testing fixed-wavelength photometric detectors used in liquid chromatography [Seite 397]
11.8 - Appendix 7. Practical aspects of using simple liquid stationary phases [Seite 409]
11.9 - Appendix 8. The practice of high-performance LC with four solvents [Seite 412]
11.10 - Appendix 9. Suppliers of well characterised polymer samples for molecular weight standards [Seite 429]
12 - List of abbreviations and symbols [Seite 430]
13 - Subject index [Seite 432]
