Principles of IVF Laboratory Practice

The evolution of IVF practice; Section I. Starting a New Laboratory & Training Protocols: 1. Establishing and equipping a new IVF laboratory; 2. Basic embryology skills and responsibilities in the IVF laboratory; 3. Sperm preparation for IVF: training protocol; 4. Oocyte pick-up for IVF: training protocol; 5. Embryologist training: embryo transfer; 6. ICSI for IVF: training protocol; 7. Embryo assisted hatching for IVF: training protocol and method; 8. Embryo biospy for IVF: training protocol; 9. Vitrification for IVF: training protocol; 10. Mouse embryo assay for quality control in the IVF laboratory; 11. Sperm survival assay for quality control in the IVF laboratory; Section II. Pre-Procedure Protocols: 12. Quality control in the IVF laboratory: continuous improvement; 13. Setup procedures for optimizing performance in the IVF laboratory; Section III. Gametes: 14. Sperm preparation for therapeutic IVF; 15. Processing surgically retrieved sperm in the IVF laboratory; 16. Cryopreservation of sperm for IVF: semen samples and individual sperm; 17. Oocyte collection for IVF; 18. In-vitro maturation of immature oocytes for IVM-IVF; 19. Oocyte grading by morphological evaluation; 20. Vitrification of oocytes for IVF; Section IV. Insemination/ICSI: 21. General insemination for IVF; 22. Conventional IVF with short co-incubation; 23. Technical aspects of ICSI for ejaculated spermatozoa; 24. Set-up and adapted protocol for piezo-ICSI; 25. Sperm selection for ICSI by morphology: MSOME/IMSI; 26. Sperm selection for ICSI by viability; 27. Artificial oocyte activation for IVF; Section V. Fertilization Assessmant: 28. Fertilization assessment in IVF and ICSI; 29. Polar body biopsy for IVF; 30. Zygote cryopreservation for IVF; Section VI. Embryo Assessment - Morphology & Beyond: 31. Embryo assessment at the pre-compaction stage in the IVF laboratory; 32. Embryo assessment in the post-compaction stage in the IVF laboratory; 33. Embryo assessment at the blastocyst stage in the IVF laboratory; 34. Trophectoderm biopsy for preimplantation genetic testing; 35. Embryo culture by Time-lapse: selection and beyond; Section VII. Embryo Cryopreservation: 36. Cryopreservation of pre-compaction embryos for IVF; 37. Cryopreservation of post-compaction embryos for IVF; 38. Safe and effective cryostorage of embryos for IVF; Section VIII. Embryo Transfer: 39. Routine embryo transfer in the IVF laboratory; Section IX. Quality Management: 40. Verification of specimen identity by double checking with a witness; 41. Quality management in the IVF laboratory: quality improvement, document and process control and adverse events; 42. Trouble-shooting in the IVF laboratory; 43. Disaster preparedness in the IVF Laboratory.