RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis. One paper describes the preparatory scale methods for the reversed-phase chromatography systems for transfer ribonucleic acids. Another paper discusses the determination of adenosine- and aminoacyl adenosine-terminated sRNA chains by ion-exclusion chromatography. One paper notes that the problems involved in preparing acetylaminoacyl-tRNA are similar to those found in peptidyl-tRNA synthesis, in particular, to the lability of the ester bond between the amino acid and the tRNA. Another paper explains a new method that will attach fluorescent dyes to cytidine residues in tRNA; it also notes the possible use of N-hydroxysuccinimide esters of dansylglycine and N-methylanthranilic acid in the described method. One paper explains the use of membrane filtration in the determination of apparent association constants for ribosomal protein-RNS complex formation. This collection is valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and investigators working with enzymes.
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-0-323-14234-2 (9780323142342)
Schweitzer Klassifikation
List of ContributorsForewordPrefaceSection I. Transfer RNA 1. Reversed-Phase Chromatography Systems for Transfer Ribonucleic Acids-Preparatory-Scale Methods 2. Miniature Reversed-Phase Chromatography Systems for the Rapid Resolution of Transfer RNA's and Ribosomal RNA's 3. Transfer RNA Isoacceptors in Cultured Chinese Hamster Ovary Cells 4. The Use of BD-Cellulose in Separating Transfer RNA's 5. Fractionation of Rabbit Liver Methionyl-tRNA Species 6. RNase Hydrolysis of Aminoacyl-sRNA to Aminoacyl Adenosine 7. Determination of Adenosine- and Aminoacyl Adenosine-Terminated sRNA Chains by Ion-Exclusion Chromatography 8. Enzymic Modification of the C-C-A Terminus of tRNA 9. Methods for Determining the Extent of tRNA Aminoacylation in Vivo in Cultured Mammalian Cells 10. The Chemical Preparation of Acetylaminoacyl-tRNA 11. The Synthesis of Oligopeptidyl-tRNA 12. Preparation of Aminoacyl-Oligonucleotides and Their Binding to Ribosomes 13. Chemical Modifications of Amino Acids Esterified to tRNA (Other Than Acylations) 14. Use of in Vitro 32P Labeling in the Sequence Analysis of Nonradioactive tRNAs 15. A New Method for Attachment of Fluorescent Probes to tRNA 16. Attachment of Cross-Linking Reagents to tRNA for Protein Affinity Labeling Studies 17. Enzymatic Synthesis of Trinucleoside Diphosphates of Known Sequence 18. Use of Purified Isoacceptor tRNAs for the Study of Codon-Anticodon Recognition in Vitro with Sequenced Natural Messenger RNA 19. Rabbit Liver tRNA Nucleotidyltransferase 20. Bacterial tRNA Methyltransferases 21. Assay for AA-tRNA Recognition by the EFTu-GTP Complex of Escherichia coli 22. Mapping the Structure of Specific Protein-Transfer RNA Complexes by a Tritium Labeling MethodSection II. Aminoacyl-tRNA Synthetases 23. Aminoacyl-tRNA Synthetase Complex from Rat Liver 24. Kinetic Techniques for the Investigation of Amino Acid:tRNA Ligases (Aminoacyl-tRNA Synthetases, Amino Acid Activating Enzymes) 25. Kinetics of Aminoacyl-tRNA Synthetases Catalyzed ATP-PP Exchange 26. Applications of Kinetic Methods to AminoacyltRNA SynthetasesSection III. Polypeptide Chain Initiation 27. Purification of Protein Synthesis Initiation Factors IF-1, IF-2, and IF-3 from Escherichia coli 28. Protein Synthesis Initiation Factors from Rabbit Reticulocytes: Purification, Characterization, and Radiochemical Labeling 29. Initiation Factors for Protein Synthesis from Wheat Germ 30. Assay of the Binding of the Ternary Complex Met-tRNAf · eIF-2 · GTP to the 40 S Ribosomal Subunit by Sucrose Gradient and CsCl Gradient Analysis 31. Prokaryotic Ribosome Binding Sites 32. Characterization of Translational Initiation Regions from Eukaryotic Messenger RNAs 33. Determination of the Number of Ribosomal Binding Sites on the RNAs of Eukaryotic Viruses 34. Methods for Analyzing Messenger Discrimination in Eukaryotic Initiation Factors 35. Isolation of Protein Kinases from Reticulocytes and Phosphorylation of Initiation FactorsSection IV. Polypeptide Chain Elongation 36. Isolation of the Protein Synthesis Elongation Factors EF-¿u, EF-Ts, and EF-G from Escherichia coli 37. Preparation of Homogeneous Escherichia coli Translocation Factor G 38. The Binding of Escherichia coli Elongation Factor G to the Ribosome 39. GTP-Dependent Binding of Aminoacyl-tRNA to Escherichia coli Ribosomes 40. Polypeptide Chain Elongation Factors from Pig Liver 41. Separation of Cytoplasmic and Mitochondrial Elongation Factors from Yeast 42. Soluble Protein Factors and Ribosomal Subunits from Yeast. Interaction with Aminoacyl-tRNA 43. Separation and Characterization of Yeast Elongation FactorsSection V. Polypeptide Chain Termination 44. Release Factors: In Vitro Assay and Purification 45. Mammalian Release Factor: In Vitro Assay and PurificationSection VI. Ribosomes 46.
