Liquid Column Chromatography

Name: Liquid Column Chromatography | A Survey of Modern Techniques and Applications
Brand: Elsevier
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A Survey of Modern Techniques and Applications

Macek(Autor*in)

K. Macek Z. Deyl J. Jan K J. Janak(Herausgeber*in)

Elsevier (Verlag)

Erschienen am 14. Mai 2014

1199 Seiten

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978-0-08-085803-6 (ISBN)

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Front Cover
Liquid Column Chromatography
Copyright Page
Contents
Foreword
Preface
PART 1: THEORETICAL ASPECTS OF LIQUID CHROMATOGRAPHY
Chapter 1. Fundamental concepts
lntroduction
Principle of chromatography
Chromatographic systems
Chromatographic techniques
Basic chromatographic quantities
Chapter 2. Basic processes in chromatography
Flow of mobile phase through a packed column
Diffusion of solute within the phases
Equilibration of solute between the phases
Chapter 3. General description of the chromatographic process
Solute mass balance in the chromatographic system
Concept of ideal linear chromatography
Concept of the theoretical plate
Dynamics of zone spreading
Chromatographic resolution
Chapter 4. Physico-chemical basis of chromatographic retention in liquid-liquid and liquid-solid systems
Interaction of solute with the phases
Thermodynamics of sorption equilibrium
Chapter 5. Gel permeation chromatography
Introduction
Principles of gel permeation chromatography
Physical basis of the separation process
Chapter 6. Fundamentals of ion-exchange chromatography
Principles and terminology
Characterization of ion exchangers
Reactions affinity and selectivity in ion exchange
lon-exchange equilibria and kinetics
Column operation and ion-exchange chromatography
Ion exclusion ion retardation. the ion-sieve process and partition chromatography on ion exchangers
Ligand-exchange chromatography
Ion exchange in non-aqueous solutions
Chapter 7. Affinity chromatography
Principles of affinity chromatography
Choice of bound affinant
General aspects of the affinant-sorbent bond
PART 2: TECHNIQUES OF LIQUID CHROMATOGRAPHY
Chapter 8. Instrumentation for liquid chromatography
Classical instrumentation for liquid chromatography
Techniques of high-efficiency liquid chromatography
Counter-current chromatography
Chapter 9. Sorbents
Rational classification
Sorbents for liquid-solid chromatography
Supports and stationary phases for liquid-liquid chromatography
Column packings for gel chromatography
Ion-exchange materials
Sorbents for affinity chromatography
Chapter 10. Mobile phases
Mobile phases for liquid-liquid chromatography
Mobile phases for liquid-solid chromatography
Mobile phases for ion-exchange chromatography
Calculation of gradients
PART 3: PRACTICE OF LIQUID CHROMATOGRAPHY
Chapter 11. Operation of a modern liquid chromatograph
Preparation of the apparatus
Sorting of sorbents according to particle size
Determination of the activity of alumina by thin-layer chromatography
Column preparation
Sample preparation and application
General comments
Chapter 12. Practice of gel chromatography
Choice of gel packing
Choice of solvent and operating temperature
Apparatus for gel chromatography
Special gel chromatographic techniques
Evaluation of gel permeation chromatographic data
Determination of molecular weights of naturally occurring macromolecular compounds by molecular sieve chromatography
Chapter 13. Practice of ion-exchange chromatography
Introduction
Choice of suitable ion exchangers
Methods for the fractionation of ion exchangers
Decantation and cycling of ion exchangers
Buffering of ion exchangers
Deaeration of ion exchangers and filling of chromatographic columns
Application of samples
Methods of elution
Calculation of flow-rates
Evaluation of fractions
Regeneration and storage of ion exchangers
Chapter 14. Practice of affinity chromatography
Preparation of the solid support with a bound affinant
Sorption conditions
Conditions for elution
Preservation of solid sorbents with a bound affinant
Chapter 15. Analytical utilization of chromatograms
Identification
Quantitation
Chapter 16. Radiochromatographic techniques
Introduction
Detectors
Detection modes
PART 4: APPLICATIONS
Chapter 17. Hydrocarbons
Introduction and general techniques
Chromatography on adsorbents
Chromatography on gels
Other methods of chromatography of hydrocarbons
Chapter 18. Alcohols and polyols
Introduction and general techniques
High-speed liquid and gel permeation chromatography of free alcohols
Chromatography of alcohols on ion exchangers
Chromatography of derivatives of alcohols and glycols
Separation of polyols and polymeric diols
Chapter 19. Phenols
Introduction
Gel chromatography
Adsorption chromatography
Ion-exchange chromatography
Chapter 20. Ethers and peroxides
Chapter 21. 0xo compounds
Introduction
Aliphatic and cyclic aldehydes and ketones
Quinones
Applications in lignin chemistry
Chapter 22. Carbohydrates
Introduction
General techniques
Mono-, oligo- and deoxy saccharides
Amino sugars
Sugar derivatives
Chapter 23. Polysaccharides
Introduction
Ion-exchange chromatography
Gel permeation chromatography
Chapter 24. Polysaccharide-protein complexes
Glycosaminoglycans (mucopolysaccharides)
Glycoproteins and glycopeptides
Chapter 25. Lower carboxylic acids
Introduction
General techniques
Separation of carboxylic acids on the basis of molecular sorption using aqueous and non-aqueous organic solvents
Ion-exchange chromatography of carboxylic acids in various aqueous acids or buffered solvent systems
High-speed ion-exchange chromatography of carboxylic acids with anion exchangers of controlled surface porosity
Other separation techniques for carboxylic acids
Chapter 26. Higher carboxylic acids
Introduction and general remarks
Separation as fatty acid derivatives
Chromatography on adsorbents in general use
Chromatography on specific adsorbents
Gel and ion-exchange chromatography
Chapter 27. Lipids
Introduction and general remarks
Separation of lipids into classes
Separation of glycerol esters and other neutral lipids
Separation of phospholipids and other polar lipids
Chapter 28. Steroids
Introduction
General techniques
Applications
Chapter 29. Terpenes
Introduction
Hydrocarbons
Ethers epoxides and furans
Esters
Aldehydes and ketones
Lactones
Alcohols
Acids
Chapter 30. Amines
Introduction
Aliphatic mono-, di- and polyamines
Aromatic mines
Aromatic mines and aliphatic polyamines in mixtures
Tryptophan metabolites
Quaternary ammonium compounds and amino alcohols
Biogenicamines
Chapter 31. Other non-heterocyclic nitrogen compounds
Introduction
Nitro compounds
Amides
Guanidine and urea derivatives
Chapter 32. Amino acids
Analytical chromatography
Preparative chromatography
Chapter 33. Amino acid derivatives
Introduction
2,4-Dinitrophenyl (DNP) amino acid derivatives
5-Dimethylaminonaphthalene-l-sulphonyl(Dns) amino acids
Hydantoins and substituted hydantoins
Miscellaneous derivatives
Chapter 34. Peptides
Introduction
Methods for the separation of peptides
Analysis of the effluent from the chromatographic column
Gel permeation chromatography
Ion-exchange chromatography
Affinity chromatography
Partition chromatography
Chapter 35. Proteins
Introduction
General rules for the separation of proteins
Gel permeation chromatography
Chromatography on glass with controlled pore size
Ion-exchange chromatography
Chromatography on hydroxyapatite and on calcium phosphate
Solubility chromatography
Technique of gel permeation chromatography in a detergent gradient
Affinity chromatography
Detection of proteins in the effluent
Chapter 36. Enzymes
Special requirements for the chromatography of enzymes
Techniques and automated analyses
Oxidoreductases
Transferases
Hydrolases
Lyases
Isomerases
Ligases
Chapter 37. Low-molecular-weight constituents of nucleic acids Nucleosides nucleotides and their analogues
Introduction
General techniques in the separation of low-molecular-weight components of nucleic acids
Automated procedures for the analysis of nucleic acid components
Individual types of nucleic acid constituents
Chapter 38. Nucleic acids
Introduction and general techniques in nucleic acid separations
Deoxyribonucleic acids
Ribonucleic acids
Polynucleotides and large oligonucleotides
Automated procedures and polynucleotide sequence analysis
Chapter 39. Alkaloids
Introduction
Preparation of samples
Techniques
Applications
Chapter 40. Other heterocyclic compounds
Introduction
Derivatives of ?-Pyrone
Anthocyans
Aflatoxins and mycotoxins
Other compounds containing heterocyclic oxygen
Porphyrins and related compounds
Indoles
Pyridine and related compounds
Polynuclear aza-heterocyclics and complex mixtures of heterocyclic compounds
Chapter 41. Organic sulphur compounds
Introduction
Sulphonic acids
Other sulphur compounds
High-speed liquid chromatography
Chapter 42. Organic phosphorus compounds
Application of column chromatography
Chapter 43. Boron compounds
General techniques
Boranes and substituted boranes
Carboranes
Ligand derivatives of boranes and carboranes
Metallocarboranes
Chapter 44. Vitamins
Introduction
Fat-soluble vitamins
Water-soluble vitamins
Chapter 45. Antibiotics
Introduction
Penicillins and cephalosporins
Carbohydrate antibiotics
Macrocyclic antibiotics
Tetracyclines and related antibiotics
Nucleoside antibiotics including polyoxins
Peptides and related antibiotics
Miscellaneous antibiotics
Chapter 46. Pesticides
Introduction and general techniques
Chlorinated pesticides and their metabolites
Phosphorus pesticides
Carbamate pesticides and their metabolites
Pyrethrins
Chapter 47. Synthetic dyes
Introduction
General techniques
Chromatography on adsorbents
Chromatography on hydrophilic gels
Chapter 48. Pigments of plastids and photosynthetic chromatophores
Introduction
Sample preparation
Chromatographic procedures
Detection
Chapter 49. Macromolecular substances and plastics
Introduction
Vinyl polymers
Rubbers
Polyolefins
Polycondensates
Copolymers
Miscellaneous polymers
Oligomers
Chapter 50. Cells and subcellular particles
Introduction
Ribosomes
Viruses
Bacteriophages
Blood cells
Cells from the spleen
Bone marrow cells
Chapter 51. Inorganic coordination and organometallic compounds
Introduction
Simple inorganic compounds
Coordination and organometallic compounds
Chapter 52. Isotopes and radioactive compounds
Isotopic effects in liquid column chromatography
Separation of radioactive substances
Subject index
List of compounds chromatographed

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