Specificity of Proteolysis presents a survey and conclusions
on the action or proteinases - enzymes which are cleaving
proteins or peptides. The specificity of proteinases which
is determined as the sequence of amino acids at the cleavage
site of a substrate, is an important criteria to choose an
enzyme as tool in protein research. Whenever one is looking
for an enzyme to act at a defined site or to give defined
cleavage products one will find comprehensive information in
this work.
Comprehensive information about more than 280 endopeptidases
which are based on the database LYSIS including a
calculation program to determine cleavage sites, is given in
the book.
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Dateigröße
ISBN-13
978-3-642-48380-6 (9783642483806)
DOI
10.1007/978-3-642-48380-6
Schweitzer Klassifikation
1 Introduction.- 2 Nomenclature and Conventions.- 2.1 EC Numbers.- 2.2 Enzyme Names.- 2.3 Enzyme and Substrate Codes.- 2.4 Subsite Nomenclature.- 2.5 Bibliography.- 3 Data Treatment.- 3.1 Data Bank LYSIS.- 3.2 Statistical Approach to Specificity.- 4 Standard Polypeptide Substrates.- 4.1 Choice of Standard Polypeptides.- 4.2 Available Data on Cleavages of Insulin Chains and Glucagon.- 4.3 Repartition of Cleavage-Susceptible Bonds.- 4.4 Binding Sites - Proposal for Fixation Site Types.- 4.5 Influence of Subsites.- 5 Essential Substrate Residues for Action of Endopeptidases.- 5.1 Basic Residue.- 5.2 Acidic Residue.- 5.3 Neutral Residue.- 5.4 Proline Residue.- 5.5 Alpha-Epsilon Peptide Bond.- 5.6 Peptidases with Occasional Endopeptidase Activity.- 5.7 Vague or Insufficient Information on Specificity.- 5.8 No Information on Bond Specificity.- 6 Comments.- 6.1 Frequently Used Proteinases and Restriction Proteinases.- 6.2 Group of Microbial Proteinases.- References.- Appendices.- A Tabular Index of LYSIS Enzyme Codes.- B Tabular Index of LYSIS Protein Codes.
