High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis.
The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution.
Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-1-4832-6202-4 (9781483262024)
Schweitzer Klassifikation
ContentsList of Contributors Conspectus Preface Evolution of Liquid Chromatography: A Historical Overview I. The Field as We See It Today II. The Beginnings III. The Dormant Period IV. The Rebirth of Chromatography V. Variants of Chromatography VI. High-Performance Liquid Chromatography VII. Postword References Practical Operation of Bonded-Phase Columns in High-Performance Liquid Chromatography I. Introduction II. Types of Bonded-Phase Chromatography III. Characteristics of Silica Gel IV. Preparation of Bonded Phases V. Phase Chain Length and Its Effect VI. Columns for Bonded-Phase Chromatography VII. Columns for Normal Bonded-Phase Chromatography VIII. Modes of Reverse-Phase Chromatography IX. Columns for Reverse-Phase Chromatography X. Ion-Pair Chromatography XI. Other Experimental Variables in Bonded-Phase Chromatography XII. Conclusions References Secondary Chemical Equilibria in High-Performance Liquid Chromatography I. Introduction II. Fundamental Concepts III. Chemical Principles of Ionic Equilibria IV. Chromatography V. Conclusion References Gradient Elution I. Introduction II. A Simplified Picture of Separation in Gradient Elution III. Experimental Considerations IV. Designing and Improving a Gradient Separation V. Theoretical Relationships VI. Conclusion List of Symbols References Index
