High-Performance Liquid Chromatography

ContentsConspectus Preface Reversed-Phase Chromatography of Proteins and Nucleic Acids: Practical Considerations I. Introduction II. Equipment Requirements III. Column Selection IV. Mobile-Phase Effects V. Other Practical Considerations VI. Hydrophobic Interaction Chromatography VII. RP-HPLC of Oligonucleotides VIII. Summary References Mixed-Interaction Stationary Phases in HPLC I. Introduction II. Concept of Mixed-Interaction Chromatography III. Biopolymer Separations IV. Low-Pressure Chromatography V. High-Performance Liquid Chromatography VI. High-Performance Mixed-Mode Chromatography VII. Conclusions References Liquid Chromatography/Electrochemistry I. Introduction II. Basic Principles of LCEC III. Practical Operational Considerations in LCEC IV. Multipotential Detection in LCEC V. Selected Applications of Liquid Chromatography/Electrochemistry References High-Performance Liquid Chromatography Separations of Large Molecules: A General Model I. Introduction II. Small-Molecule Retention III. Small-Molecule Band Broadening IV. Large-Molecule HPLC: Retention Relationships V. Large-Molecule HPLC: Band Broadening VI. Separation and Resolution VII. Non-Well-Behaved Samples VIII. The Design of Columns for Macromolecular HPLC IX. Other Models of Macromolecular HPLC X. Conclusions Appendix I. Use of "Normal versus Superficial" Mobile-Phase Velocity: Effect on C Term of Knox Equation Appendix II. Examples of Calculations by the Model of Table VIII Glossary of Symbols References Index