High-Performance Liquid Chromatography: Advances and Perspectives, Volume 2 presents the fundamental aspects of high-performance liquid chromatography, laboratory technique for chemical analysis with a wide range of applications. The book consists of three chapters discussing the optimization of the column and the operating conditions of the chromatographic system; use of polar adsorbents and nonpolar eluents; and reversed-phase chromatography, the main branch of high-performance liquid chromatography. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good reference material.
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-1-4832-6183-6 (9781483261836)
Schweitzer Klassifikation
Contents List of Contributors Conspectus Preface Contents of Volume 1Optimization in Liquid Chromatography I. Introduction II. Resolution and Efficiency III. Analysis Time, Flowrate, and Pressure IV. Flow Velocity and Column Efficiency V. What to Optimize in Liquid Chromatography VI. Equations Used for Optimization VII. Ways to Achieve 5000 Plates in 5 Minutes VIII. Column Performance at Minimum Inlet Pressure IX. Critical Minimum Pressure X. Achievement of Fastest Analyses XI. Practical Considerations XII. Equipment Specifications XIII. Conclusions References Liquid Chromatography on Silica and Alumina as Stationary Phases I. Introduction II. Stationary Phases III. Role of the Eluent IV. Effect of Sample Structure on Retention V. Anisocratic Analysis VI. Theory of Adsorption Chromatography VII. Selected Applications VIII. Prospects References Reversed-Phase Chromatography I. Introduction II. The Stationary Phase III. The Mobile Phase IV. Effect of Temperature V. Theory of Reversed-Phase Chromatography VI. Modulation of Selectivity by Secondary Equilibria VII. Physicochemical Measurements VIII. Selected Analytical Applications Notation References Index Erratum