Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry.
The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes.
The book will be of great use to students, researchers, and practitioners of biochemistry.
Sprache
Verlagsort
ISBN-13
978-1-4831-8836-2 (9781483188362)
Schweitzer Klassifikation
List of ParticipantsIntroductory RemarksPart I Gel Matrices, Coupling Methods and Charge Transfer Chromatography Gel Matrices, Coupling Methods and Charge Transfer ChromatographyPart II Affinity Chromatography of Enzymes Enzyme Kinetics and Affinity Chromatography (This Paper was Not Submitted for Publication in this Volume.) Affinity Chromatography of Enzymes Affinity Chromatography on Immobilized Coenzymes Immobilized Adenine Coenzymes in General Ligand Affinity Chromatography and Their Use as Active Coenzymes Dissociation Constants of Phosphorylases to Oligo- and Polyglucans Studied by the Affinity Electrophoresis Affinity Chromatography and Conformational Isomers of dCMP-Aminohydrolase Optimization of Conditions for the Affinity Chromatography of Human Enterokinase Affinity Chromatography of Brain Glutamate Decarboxylase (EC 4.1.1.15) using Immobilized Pyridoxal Phosphate Affinity Chromatography of Tryptophan Synthase from E. Coli Affinity Chromatography of the Pyruvate Dehydrogenase Complex Using TPP as a Ligand Temperature Dependence, Activation Energy and Enthalpy Change of the Binding Process of UDP-Galactose 4'-Epimerase to Its Immobilized Substrate Affinity Chromatography of Chymotrypsin on Soybean Trypsin Inhibitor Sepharose: Applications in Genetics and Nuclide Labeling Reversible Immobilization of Vibrio Cholerae Neuraminidase by Biospecific Sorption Affinity Chromatography of Carbohydrate Binding Proteins. Preparation and Use of Some Potential Ligands Direct Coupling of Reducing Oligosaccharides to Aminohexyl-Sepharose: Purification of a,a-Trehalase from Artemia Salina Purification of Pectinesterase from Aspergillus Niger by Chromatography on an Amide Derivative of the Cross-Linked Pectate A Chymotrypsin Inhibitor from Adult Culex Pipiens Isolated by Affinity Chromatography Separation of Proteinases and in Particular of Clostripain and Collagenase by Affinity Chromatography Synthesis of Imidazole Containing Matrices (Polyhydroxyethylmethacrylates, Polysaccharides) and Its Application in Affinity Chromatography Purification of Neutral Proteases from Leucocytes on Protamine-SepharosePart III Hydrophobic Interaction Chromatography Homologous Series of Hydrocarbon-Coated Agarose in Hydrophobic Chromatography Adsorption of Proteins at High Salt Concentrations on Hydrophobically Interacting Matrices The Binding of Myoglobin and Hemoglobin to Hydrophobically Substituted Sepharoses On the Purification of Bacterial Tyrosine Decarboxylase Improved Synthesis of a Fatty Acid-Agarose Complex for Affinity Chromatography of Serum AlbuminPart IV Matrix-bound Antigens and Antibodies Matrix-Bound Antigens and Antibodies Comparison of Sepharose and Cellulose as a Support for Antibody and Antigen Approach to the Sequence Analysis of Proteins with HLA Activity by Affinity Chromatography Bio- and Immunospecific Affinity Chromatography of Plasma Membrane Antigens from Human Kidney and Renal Cell Carcinoma The Purification of Melanoma Antigen from Human Urine Benzylpenicilloyl (BPO)-Specific Affinity and Subsequent Ion Exchange Chromatography: Characterization of Antibody Subtractions Immunosorbent Separation of IgG and IgM for the Radio-immunoassay of Specific Antibodies Elution by Glucose of Bovine Immunoglobulins Adsorbed on Cross-Linked Dextran Purification of Enzyme-Labeled Conjugate by Affinity Chromatography Killed Staphylococcal Aureus Cells as Support for Antibody Mediated Affinity Chromatography The Use of Affinity Chromatography for Purification of Enzyme-Antibody Conjugates Purification of DD-Carboxypeptidases from Streptomyces Strains R6l and K15 by Antigen-Antibody Affinity Chromatography Selective Removal of Fast Dissociating Antibodies from a High-Affinity Thyroxine Antiserum to be Used in Radio-Immunoassay Adsorption and Elution Characteristics of P.
