High-Performance Liquid Chromatography of Proteins and Peptides
Proceedings of the first International Symposium
Erschienen am 2. Dezember 2012
284 Seiten
978-0-323-15770-4 (ISBN)
Beschreibung
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.
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ContributorsPrefaceHigh-Performance Ion-Exchange Chromatography of Proteins: The Current StatusAnalysis of Peptic Fragmentation of Human Immunoglobulin G Using High-Performance Liquid ChromatographyCord Blood Screening for Hemoglobin Disorders by High-Performance Liquid ChromatographyA Simple and Rapid Purification of Commercial Trypsin and Chymotrypsin by Reverse-Phase High-Performance Liquid ChromatographyAdaptation of Reverse-Phase High-Performance Liquid Chromatography for the Isolation and Sequence Analysis of Peptides from Plasma Amyloid P-ComponentCharacterization of Human Alcohol Dehydrogenase Isoenzymes by High-Performance Liquid Chromatographic Peptide MappingPurification of Glycopeptides of Human Ceruloplasmin and Immunoglobulin D by High-Pressure Liquid ChromatographyHigh-Performance Size-Exclusion Chromatography of Hydrolyzed Plant ProteinsThe Isolation of Peptides by High-Performance Liquid Chromatography Using Predicted Elution PositionsThe Application of High-Performance Liquid Chromatography for the Resolution of Proteins Encoded by the Human Adenovirus Type 2 Cell Transformation RegionThe Importance of Silica Type for Reverse-Phase Protein SeparationsVariables in the High-Pressure Cation-Exchange Chromatography of ProteinsApplication of High-Performance Liquid Chromatography to Competitive Labeling Studies: The Chemical Properties of Functional Groups of GlucagonPurification by Reverse-Phase High-Performance Liquid Chromatography of an Epidermal Growth Factor-Dependent Transforming Growth FactorPreparative Size-Exclusion Chromatography of Human Serum Apolipoproteins Using an Analytical Liquid ChromatographUse of Reverse-Phase High-Performance Liquid Chromatography in Structural Studies of Neurophysins, Photolabeled Derivatives, and Biosynthetic PrecursorsPurification of Radiolabeled and Native Polypeptides by Gel Permeation High-Performance Liquid ChromatographyNonideal Size-Exclusion Chromatography of Proteins: Effects of pH at Low Ionic StrengthFactors Influencing Chromatography of Proteins on Short Alkylsilane-Bonded Large Pore-Size SilicasHigh-Performance Liquid Chromatography Analysis in the Synthesis, Characterization, and Reactions of NeoglycopeptidesUse of Size-Exclusion High-Performance Liquid Chromatography for the Analysis of the Activation of ProthrombinHigh-Performance Liquid Chromatography of Proteins: Purification of the Acidic Isozyme of Adenylosuccinate Synthetase from Rat LiverApplication of High-Performance Liquid Chromatographic Peptide Purification to Protein Microsequencing by Solid-Phase Edman DegradationMeasurement of Endogenous Leucine Enkephalin in Canine Caudate Nuclei and Hypothalami with High-Performance Liquid Chromatography and Field-Desorption Mass SpectrometryFluorescent Techniques for the Selective Detection of Chromatographically Separated PeptidesHigh-Performance Liquid Chromatographie Purification of Peptide Hormones: Ovine Hypothalamic Amunine (Corticotropin Releasing Factor)Isolation and Purification of Escherichia coli Heat-Stable Enterotoxin of Porcine Origina-H-Acetyl-ß-Endorphin1-26 from the Neurointermediary Lobe of the Rat Pituitary: Isolation, Purification, and Characterization by High-Performance Liquid ChromatographyIndex
