UHPLC in Life Sciences

1.1. Trends in HPLC;

1.2. Comparison of Chromatographic Techniques and Supports;

1.3. Constructing Kinetic Plots;

1.4. History of the Kinetic Plot Method;

1.5 Unification of the Isocratic and Gradient Kinetic Plot Method;

1.6. Relation between the Kinetic Performance under Isocratic and Gradient Elution Conditions;

1.7. Influence of the Test Conditions on the Obtained Kinetic-Performance Limit Curve;

1.8. Some Reflections on Recent Trends in Liquid Chromatography using the Kinetic Plot Method;

1.9. Conclusions;

Acknowledgements;

References;

2.1 UHPLC Instrumentation;

2.2 UHPLC Columns;

3.1 Introduction;

3.2 Qualitative Transfer from HPLC to UHPLC;

3.3 Normative Context for the HPLC to UHPLC Transfer;

3.4 Validation of UHPLC Methods and Equivalence of the HPLC-UHPLC Methods;

3.5 Conclusions;

3.6 References;

4.1 Introduction;

4.2 High throughput and high resolution in HT-UHPLC;

4.3 Limitations of HTLC and HT-UHPLC;

4.4 Advantage of high temperature in life science analysis;

4.5 HT-UHPLC in comprehensive on-line two-dimensional liquid chromatography (LC x LC);

4.6 Conclusion;

5. Comparison of the performance of totally porous and core-shell particles;

5.1. Introduction;

5.2. Column performance;

5.3. Possibilities of recent core-shell technology;

5.4. Particle size distribution and roughness of core-shell particles;

5.5. Loading capacity of core-shell particles;

5.6. Limited efficiency when core-shell particles packed in narrow-bore columns;

5.7. Extra column effects, contribution to band broadening;

5.8. Performance of core-shell and totally porous particles in isocratic elution mode;

5.9. Performance of core-shell and totally porous particles in gradient elution mode;

5.10. Conclusion;

6.1 Introduction;

6.2 Analytical Conditions for performing HILIC.;

6.3 Applications of HILIC in UHPLC.;

6.4 References.;

7.1 Introduction;

7.2 Selection of ionization techniques;

7.3 Overview of mass analyzers and their main features;

7.4 New developments in mass spectrometry applicable in UHPLC/MS;

7.5 Conclusions;

8.1 Introduction;

8.2 Solubility;

8.3 Ionization;

8.4 Lipophilicity;

8.5 Permeability;

8.6 Conclusion;

8.7 References;

9.1 Introduction;

9.2 UHPLC in Bioanalysis;

9.3 Sample preparation for UHPLC in bioanalysis;

9.4 Conclusions;

10.1 Introduction;10.2 GC-MS Analyses;

10.3 LC-MS(/MS) Analyses;

10.4 Application of UHPLC-MS(/MS) for Drug Testing in Sports;

10.5 Conclusion;

References;

11.1 Introduction;

11.2 Use of UHPLC for the Analysis of Seized Drugs;

12.1. Pharmaceuticals as environmental contaminants;

12.2. Analysis of pharmaceuticals in environmental samples;

12.3. Occurrence of pharmaceuticals in environmental and wastewater samples;

12.4. Conclusions;

Acknowledgements;

References;

13.1 Introduction;

13.2 Multiple Facets of UHPLC in NP research;

13.3 Fast Targeted Analysis;

13.4 Fast Non-Targeted Analysis, Fingerprinting, and Metabolomics;

13.5 High-Resolution Profiling and Metabolite ID;

13.6 Conclusion;

Acknowledgments;

References;

14.1. Introduction;

14.2. Pre-analysis Considerations: Protocol Design, Sample Collection, Storage and Preparation;

14.3. Sample Preparation for Serum and/Plasma;

14.4. Collection and Storage of Urine Samples;

14.5. UHPLC-MS-Based Metabolite Profiling;

14.6. Applications of UHPLC-MS to Human Metabolic Profiling Studies;

14.7 Current Challenges for UHPLC-MS in Global metabolic profiling Studies;

14.8. Conclusions