Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry

Name: Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry
Brand: Elsevier
Price: 250.0 EUR
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Desiderio D. M. Desiderio(Autor*in)

Elsevier (Verlag)

Erschienen am 14. Mai 2014

254 Seiten

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Front Cover
Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry
Copyright Page
Contents
List of Abbreviations
Preface
Chapter 1. Introduction
1.1. Objective
1.2. Main theme
References
Chapter 2. Neuropeptides
2.1. Introduction
2.2. Opioid peptides
2.3. Opiates
2.4. Neurons
2.5. Neuroregulatory peptides
2.6. Peptide distribution
2.7. Peptide degradation
2.8. Brain distribution of peptides
2.9. Dynorphin
2.10. Opioid receptors
2.11. HPLC of peptides
2.12. Modified enkephalins and endorphins
2.13. Urinary peptides
2.14. CSF peptides
2.15. Peptides in nutrition
2.16. Synthesis of peptides
2.17. Other neuropeptides
2.18. Concentrations of endogenous peptides
2.19. Summary
References
Chapter 3. Biochemical Sampling Techniques
3.1. Introduction
3.2. Time factors
3.3. Post-translational modifications of peptides
3.4. Animal size
3.5. Species differences
3.6. Method of sacrifice
3.7. Internal brain structures
3.8. Neuropeptidase activity
3.9. Summary
References
Chapter 4. Reversed Phase Chromatography of Peptides
4.1. Introduction
4.2. Difference between reversed phase and normal phase chromatography
4.3. Anatomy of a reversed phase chromatography column
4.4. Chemical synthesis of bonded phases
4.5. Modes of interaction between column and solute
4.6. Silica gel
4.7. Organic modifiers
4.8. Buffers
4.9. Low resolution mini-chromatographic columns
4.10. Peptide separations
4.11. High resolution analytic RP-HPLC columns
4.12. HPLC detectors
4.13. RP-HPLG of biologic peptides
4.14. Other aspects
4.15. Summary
References
Chapter 5. Analytical Measurements of Endogenous Peptides
5.1. Introduction
5.2. Radioimmunoassay of peptides
5.3. Bioassay
5.4. Radioreceptor analysis
5.5. Summary
References
Chapter 6. Mass Spectrometry of Peptides
6.1. Introduction
6.2. EI mass spectrometry of peptides
6.3. Examples of EI mass spectra of chemically derivatized peptides
6.4. Chemical ionization mass spectrometry
6.5. Field desorption-mass spectrometric studies of peptides
6.6. Quantitative field desorption mass spectrometry
6.7. Fast atom bombardment mass spectrometric studies of peptides
6.8. Other FAB MS techniques
6.9. CAD MS
6.10. Scanning methods to obtain metastable transitions
6.11. Computer techniques
6.12. 252Californium plasma desorption mass spectrometry
6.13. Fourier transform MS
6.14. Summary
References
Chapter 7. Measurement of Endogenous Biological Peptides with Mass Spectrometry
7.1. Introduction
7.2. Neuropeptide measurements
7.3. Basic principles of analytical measurement of peptides by mass spectrometry
7.4. Mass spectrometric measurement of endogenous peptides
7.5. Stable isotope-incorporated peptide internal standards
7.6. Types of mass spectrometry measurement modes
7.7. Examples of analytical measurements of endogenous enkephalin peptides
7.8. Comparison of RIA and MS values
7.9. Scheme for endogenous peptide amounts
7.10. Metabolic profiles of peptides in tooth pulp
7.11. Peptide extraction at neutral pH
7.12. Comparison of FD and FAB MS measurement methods
7.13. Summary
References
Chapter 8. Instrumental Developments
8.1. Introduction
8.2. Conversion dynode
8.3. Higher mass capabilities
8.4. Higher sensitivity
8.5. Negative ions
8.6. On-line combination of liquid chromatography and mass spectrometry
8.7. Summary
References
Subject Index

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