The methods described in the second edition on
Legionella
are for the study of distinct features of
L. pneumophila
. Chapters guide readers through ecology and physiology of
legionella, legionella
genetics, cellular microbiology of
legionella,
biochemical assays to study
legionella
effectors and enzymes, immunity and host response against
legionella,
metagenomics, proteomics, and host microbiomes of
legionella.
Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Legionella: Methods and Protocols, Second Edition
aims to be useful for scientists studying
Legionella
, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
50
24 s/w Abbildungen, 50 farbige Abbildungen
XXVI, 476 p. 74 illus., 50 illus. in color.
ISBN-13
978-1-4939-9048-1 (9781493990481)
DOI
10.1007/978-1-4939-9048-1
Schweitzer Klassifikation
Analysis of Iron Requirements and Siderophore Production.- The Pathometabolism of Legionella Studied by Isotopologue Profiling.- Quantification of Viable but Non-culturable Cells of Legionella pneumophila.- Selection of Legionella Virulence-related Traits by Environmental Protozoa.- Migration of Acanthamoeba castellanii through Legionella Biofilms.- Scar-free Genome Editing in Legionella pneumophila.- Querying Legionella Genomes using Transposition-sequencing.- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq.- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae.- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium.- Sorting of Phagocytic Cells Infected with Legionella pneumophila.- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment.- Mitochondrial Dynamics and Activity in Legionella-Infected Cells.- Perturbation of Legionella Cell Infection by RNA Interference.- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography.- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins.- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila.- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification.- Inflammasome Activation in Legionella-infected Macrophages.- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila.- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence.- Dictyostelium Host Response to Legionella Infection: Strategies and Assays.- 6.1.3 The Caenorhabditis elegans model of Legionella infection.- The Mouse as a Model for Pulmonary Legionella Infection.- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples.- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients.- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry.
