Histones and Other Nuclear Proteins

PrefaceAcknowledgments Introduction Classification of the Nuclear Proteins The Role of Proteins in Control of the Genome Repressor Substances History of the Nuclear Proteins The Histones The Acidic Nuclear Proteins Enzymes of the Nucleus ReferencesPart I The Basic Nuclear Proteins Chapter I. The Protamines General Discussion Primary Structure of Protamines Functions of the Protamines References Chapter II. Types, Composition, and Number of Histones Classification of the Histones Amino Acid Composition of Histones Amino Acid Composition of Products of Recent Fractionation Procedures NH2-Terminal Amino Acid Analysis of Histones Physical Methods for Classification of the Histones Sedimentation Analysis of the Histones The Number of Histones Distribution of Histones in Nature References Chapter III. Isolation of the Histones Introduction Isolation of Nuclei Direct Isolation of Nucleoproteins Fractionation of the Histones Older Methods for Fractionation of the Histones Chromatography of the Histones Electrophoresis of Histones Sedimentation of the Histones References Chapter IV. Spatial Relationships between DNA and Histones Introduction Relationship of Histones to Chromosome Structure Models of the Structure of the Chromosome Special Linkages of Histones and DNA References Chapter V. Functions of the Histones Introduction The Concept That Histones are Gene Modifiers Suppression of Biosynthesis of RNA Effects of Histones on DNA Synthesis Numerical Relationships between the Suppressive Potentialities of Histones and Loci of DNA Histones of Specialized Tissues Histones of Cancer Cells Histones as Ribonucleases Other Potential Functions of the Histones References Chapter VI. The Primary Structure of Histones Introduction Early Studies on Peptides of the Histones Peptides of the N-Proline Histone Fraction Elementary Amino Acid Analysis of Peptides of the N-Proline Histone Fraction Sequential Analysis of Amino Acids in the N-Proline Histone Fraction The 2a or N-Acetylalanine Histone Fraction References Chapter VII. Metabolism of Histones Introduction Synthesis of Histones in Dividing Cells Labeling of Histones in Vivo Labeling of Specific Histones in Vivo Fraction of Total Histone Turning Over in One Day When Are Histones Synthesized in the Cell Cycle? Catabolism of Histones ReferencesPart II The Acidic Nuclear Proteins and the Nuclear Enzymes Chapter VIII. The Acidic Nuclear Proteins Introduction Quantitative Studies on Acidic Nuclear Proteins Nuclear Ribonucleoproteins Physical and Chemical Properties of the Acidic Nuclear Proteins Subfractionation of the Acidic Nuclear Proteins Amino Acid Analysis of the Acidic Nuclear Proteins NH2-Terminal Amino Acids of the Acidic Nuclear Proteins Biosynthesis of the Acidic Nuclear Proteins Effects of Antitumor Agents on the Biosynthesis of Acidic Nuclear Proteins References Chapter IX. Nuclear Enzymes Introduction DPN Synthetase or Pyrophosphorylase Enzymes Involved in Synthesis of Nucleic Acids RNA Polymerase Nuclear Ribonucleases Oxidative Enzymes in the Nucleus Enzymes of Protein Synthesis Hydrolytic Enzymes of Nuclei Other Nuclear Enzymes ReferencesAuthor IndexSubject Index