High Pressure Liquid Chromatography: Biochemical and Biomedical Applications covers basic information on high pressure liquid chromatography in a simple and concise manner. It describes high pressure liquid chromatography, encompassing the method's history and advantages. The book explains the instrumentations, experimental methods, peak identification, quantitation, and applications of high pressure liquid chromatography. It also discusses the pitfalls likely to be encountered in utilizing such method. This reference serves as an introductory book for all those who are unfamiliar with high pressure liquid chromatography. This book can also be used as a reference for those who are currently using the technique. It can also aid in promoting the use of high pressure liquid chromatography in all biochemical and biomedical researches.
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
ISBN-13
978-0-323-16005-6 (9780323160056)
Schweitzer Klassifikation
Preface AbbreviationsChapter 1. Introduction I. Description II. History III. Comparison of Chromatographie Methods IV. Advantages of High Pressure Liquid Chromatography Chapter 2. Instrumentation I. Hardware II. Pumps III. Detectors IV. Columns V. Recorders, Fraction Collectors, and Integrators Chapter 3. Experimental Methods I. Standardization of Conditions II. Operating Parameters III. Preparation of Sample IV. Use of Fraction Collector V. Automation VI. Column Maintenance VII. Instrument MaintenanceChapter 4. Identification of Peaks I. Use of Retention Data II. Use of Internal Standards III. Isotopic Labeling IV. Collection and Characterization of Eluent Peaks V. Enzymic Peak-Shift VI. DerivatizationChapter 5. Quantitation I. Chromatography II. Integration III. Calculation IV. Statistical Treatment of DataChapter 6. Applications I. General Applications II. Specific ApplicationsBibliographyAuthor IndexSubject Index
