Principles and Reactions of Protein Extraction, Purification, and Characterization

EXTRACTION OF PROTEIN Preparation of Buffers for Protein Extraction Use of Protease Inhibitors in Protein Extraction Use of Detergents in Protein Extraction Chemical Lysis for Protein Extraction Mechanical Lysis for Protein Extraction Preparation of Extract from Prokaryotes Extraction of Recombinant Protein from Bacteria Preparation of Extracts from Yeast Preparation of Extracts from Eukaryotes Preparation of Extracts from Plants Preparation of Membrane Extracts ESTIMATION OF PROTEIN Ultraviolet Absorption Methods Colorimetric Methods Fluorescent Methods ELECTROPHORETIC ANALYSES OF PROTEIN Driving Force of Electrophoresis Polyacrylamide Gel Electrophoresis (PAGE) Isoelectric Focusing (IEF) Two-Dimensional (2-D) Gel Electrophoresis Western Blotting Capillary Electrophoresis PURIFICATION OF PROTEIN General Consideration and Purification Strategy Non-Chromatographic Purification of Proteins Chromatographic Purification of Proteins ANTIBODIES: STRUCTURES, INTERACTIONS AND PRODUCTION Immune System and Antibody Response Structure of Antibodies Antigen-Antibody Interactions Production oof Antibodies Development of Monoclonal Antibodies Purification of Antibodies ANTIBODY LABELING, ANTIBODY DETECTION AND IMMUNOCHEMICAL TECHNIQUES Methods of Labeling Antibodies Detection of Antibody Binding Immunochemical Techniques: a Wide Range of Sensitive Assays for Detecting and Quantitating (Semi) Antigen-Antibody Reactions Immunoprecipitation Immunohistochemistry Flow Cytometry PURIFICATION OF GLYCOPROTEINS AND ANALYSES OF THEIR OLIGOSACCHARIDES Diagrams and Stereochemistry of Monosaccharides and Oligosaccharides Purification of Glycoproteins Release of Oligosaccharides From Glycoproteins Preparation of Glycopeptides by Proteolytic Cleavage Estimation of Carbohydrates Chromatographic Separation and Detection of Sugars Electrophoretic Separation Glycan Detection/Differentiation by Lectins Analyses of Carbohydrate Linkage