This book is designed as an introductory guide for students and laboratory technicians in instrumental analysis, analytical chemistry, biochemistry, biotechnology, and molecular biology who want to learn how to perform new liquid chromatography methods. Over 34 self-contained, practical experiments are presented. Objectives and basic information introducing the method are given at the outset of each experiment; notes included at the end offer practical advice and insights that have proven useful for people performing an experiment for the first time. Several experiments deal with the general aspects of HPLC as a tool for qualitative and quantitative analysis and are designed to help students develop the required skills for this type of work. Other experiments discuss efficient approaches for methods development and developing preparative scale separations.
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Maße
Höhe: 279 mm
Breite: 216 mm
Gewicht
ISBN-13
978-0-8493-0177-3 (9780849301773)
Schweitzer Klassifikation
INTRODUCTION. Purpose. How to Use this Book. Basic Chromatographic Theory. General Experimental Techniques. Qualitative Analysis. Quantitative Analysis. REVERSED PHASE EXPERIMENTS. Determination of Caffeine in Beverages by the Methods of Internal and External Standards. A Study of Selectivity and Mobile Phase Composition. Isocratic Mobile Phase Optimization in Reversed Phase Liquid Chromatography. Resolution and Peak Purity. Comparison of Gradient Elution and Isocratic Methods. Determination of Ethyl Vanillin in Chocolate. Fluorescence Detection of Polycyclic Compounds. Monitoring Reaction Rates with an Autosampler. Preparation of a van Deemter Plot. Packing and Evaluating a Reversed Phase Column. Effects of Sample Size on Chromatographic Behavior. Trace Analysis of Carbamate Pesticides Using Electrochemical Detection. Tryptic Mapping of Egg White Lysozyme. Resolution and Quantitation of Amino Acids. The Amino Acid Sequence of Angiotensin. Chiral Separation of Amino Acids by Ligand Exchange Chromatography. A Study of the Electronic Integration of Chromatographic Peaks. NORMAL PHASE EXPERIMENTS. Isocratic Mobile Phase Optimization in Normal Phase Liquid Chromatography. Analysis of Transition Metal Chelates. Determination of Sugars in Foods and Beverages on Dynamically Modified Silica with a Refractive Index Detector. SIZE EXCLUSION EXPERIMENTS. Determination of the Molecular Weight of a Protein. Effects of Sample Dilution and Eluant on Chromatographic Behavior of Proteins. ION EXCHANGE AND ION PAIR EXPERIMENTS. Ion Exchange Chromatography of Organic Acids in White Wine. Qualitative Analysis for FD&C Dyestuffs in Foods and Cosmetics. Indirect Photometric Determination of Anions in Drinking Water. PREPARATIVE SCALE EXPERIMENTS. Preparative Scale Separation of Phthalate Esters. Purification of b-Tocopherol from a Vitamin E Mixture in Milligram Quantities. APPENDICES. Appendix A: Mobile Phase Composition Graphs. Appendix B: General Information.
