AMINO ACIDS AND POLYPEPTIDES. INTRODUCTION. Amino Acids. Peptides. SAMPLE PREPARATION. Sample Sources, Sampling, Storage. Sample Processing. Analyte Release. Extraction, Enrichment, Purification. SEPARATION MODES. Amino Acids. Peptides. DETECTION AND DERIVATIZATION. UV Detection. Fluorometric Detection. Electrochemical Detection. SELECTED EXAMPLES (ANALYTICAL PROBLEMS-SELECTED APPLICATIONS). References. BIOPOLYMERS. INTRODUCTION. Chemistry of Proteins. Chemistry of Polynucleotides. PROBLEMS OF SAMPLE PREPARATION. Prefractionation. Solubilization. Sample Concentration. Desalting. Filtration. Affinity Chromatography. SEPARATION MODE SELECTION. Gel Filtration. Ion-Exchange Chromatography. Reversed-Phase Chromatography. METHODS TO IMPROVE SEPARATION. Special Stationary Phases. Hydrophobic Interaction Chromatography. Displacement Chromatography of Biopolymers. Related Techniques for Biopolymer Separation. PROBLEMS OF DERIVATIZATION, DETECTION, AND QUANTITATION. Derivatization. Detection and Quantitation. ANALYTICAL PROBLEMS-SELECTED APPLICATIONS. Protein Analysis. Polynucleotides. References. ALKALOIDS. INTRODUCTION. Indole-Type Alkaloids. Opium Alkaloids. Tropane Alkaloids. Other Alkaloids. PROBLEMS OF SAMPLE PREPARATION. Determination of Alkaloids and Their Metabolites in Biological Samples. Determination of Alkaloids in Plants. Determination of Alkaloids in Pharmaceutical Formulations. SEPARATION PROBLEMS. Separation of Highly Polar and Polar Alkaloids. Separation of Closely Related Alkaloids and Alkaloid Isomers. Separation of Structurally Different Alkaloids in Mixture. SEPARATION MODE SELECTION. STATIONARY PHASE SELECTION. MOBILE PHASE SELECTION. Indole-Type Alkaloids. Opium Alkaloids. Tropane Alkaloids. Miscellaneous Other Alkaloids. METHODS TO IMPROVE SEPARATION. Application of Various Amines as Mobile Phase Additives. Ion-Pair Chromatography in Reversed-Phase and Normal-Phase Modes. Chiral Separations. PROBLEMS OF DERIVATION, DETECTION, AND QUANTITATION. Problems of Derivation. Problems of Detection and Quantitation. ANALYTICAL PROBLEMS-SELECTED APPLICATIONS. Analysis of Starting Raw Materials. In-Process Control in the Synthesis of Alkaloids. Purity Testing and Assay of Bulk Alkaloids. Analytical Control of Alkaloid Formulations. Stability Testing. Determination of Alkaloids in Biological Fluids. References. ANTIBIOTICS. INTRODUCTION. PROBLEMS OF SAMPLE PREPARATION. Carbohydrate Antibiotics. Macrocyclic Lactones. Quinone Antibiotics. Amino Acid and Peptide Antibiotics. Other Antibiotics. SEPARATION MODE SELECTION. Carbohydrate Antibiotics. Macrocyclic Lactone Antibiotics. Quinone Antibiotics. Amino Acid and Peptide Antibiotics. METHODS OF IMPROVING SEPARATION. PROBLEMS OF DERIVATIZATION, DETECTION, AND QUANTITATION. Carbohydrate Antibiotics. Macrocyclic Lactones. Quinone Antibiotics. Amino Acid and Peptide Antibiotics. SELECTED APPLICATIONS. References. PROSTAGLANDINS. INTRODUCTION. PGs Containing Saturated Cyclopentane Ring Systems (PGF-, PGD- and PGE-Derivatives) (Type I). PGs Containing Cyclopentene Ring System (Type II). Prostacyclin Derivatives (PGI Series) (Type III). PROBLEMS OF SAMPLE PREPARATION. SEPARATION PROBLEMS. Separation of Closely Related PGs. Separation of PG Isomers. SEPARATION MODE SELECTION. STATIONARY PHASE SELECTION. MOBILE PHASE SELECTION. Type I and II PGs. Type III PGs (Prostacyclins). METHODS TO IMPROVE SEPARATION. Application of Cyclodextrin Inclusion Complex Formation. Separations by Using Silver-Modified Stationary or Mobile Phases. PROBLEMS OF DERIVATIZATION, DETECTION, AND QUANTITATION. Problems of Derivatization. Problems of Detection and Quantitation. ANALYTICAL PROBLEMS-SELECTED APPLICATIONS. Identification of Anti-Inflammatory Drugs Acting by Suppression of PG Synthesis. Assay of PGs in Bulk Drug Substance and Formulations. Purification of PGs by Preparative HPLC. References. SUGARS AND SUGAR DERIVATIVES. INTRODUCTION. Sugars in General Classification of Carbohydrates. SAMPLE PREPARATION IN THE CHROMATOGRAPHIC ANALYSIS OF CARBOHYDRATES. The Main Fields of Chromatographic Separations. CHROMATOGRAPHIC METHODS. Chromatographic Methods in General. HPLC in General. HPLC Analysis of Different Types of Carbohydrates. METHODS TO IMPROVE SEPARATIONS. DERIVATIZATION IN HPLC. EMPLOYMENT OF HPLC IN SUGAR ANALYSIS. References. STEROIDS. INTRODUCTION. PROBLEMS OF SAMPLE PREPARATION. Determination of Steroids and Their Metabolites in Biological Samples. Determination of Steroids in Plants. Determination of Steroids in Pharmaceutical Formulations. SEPARATION PROBLEMS. Separation of Highly Polar Steroids. Separation of Medium Polar Steroids. Separation of Nonpolar Steroids. SEPARATION MODE SELECTION. STATIONARY PHASE SELECTION. MOBILE PHASE SELECTION. METHODS TO IMPROVE SEPARATION. PROBLEMS OF DERIVATIZATION, DETECTION, AND QUANTITATION. Problems of Derivatization. Problems of Detection and Quantitation. ANALYTICAL PROBLEMS-SELECTED APPLICATIONS. Drug Discovery. Impurity Profiling. Analysis of Starting Raw Materials. In-Process Control in Steroid Synthesis. Purity Testing and Assay of Bulk Steroids. Analytical Control of Steroid Formulations. Stability Testing. Determination of Steroids in Biological Fluids. References. SULFONAMIDES. INTRODUCTION. SAMPLE PREPARATION. Pretreatment of Pharmaceuticals. Pretreatment of Biological Materials. SEPARATION MODE SELECTION. Chemometric Methods for Separation Mode Selection. Selection of the HPLC Methods and Separation Conditions. COUPLED METHODS TO IMPROVE SEPARATIONS. Determination of Sulfonamides in Biological Fluids by HPLC-MS-MS. Determination of Sulfonamides by Supercritical SFC-MS. Automated HPLC (CF-LC) Procedure for Monitoring Sulfonamide Residues. DERIVATIZATION, DETECTION, AND QUANTITATION. SELECTED APPLICATIONS. Assay of Different Pharmaceuticals. Assay of Drug Residues in Different Biological Samples. References.
360 pp., 7x10, 1990, ISBN 8493-6999-1.
