Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes and Philip Ingham; nematode embryos, Lois G. Edgar and William B. Wood; embryos and larvae of invertebrate deuterostomes, Nicholas D. Holland and Linda Z. Holland; zebrafish embryos, Rachel M. Warga; Xenopus, Ariel Ruis I. Altaba; avian embryos, Claudio D. Stern; post-implantation of mammalian embryos, Gillian M. Morriss-Kay; simple tips for photomicrography of embryos, Claudio D. Stern. Part 2 Experimental embryology: fate maps and cell lineage analysis, Ariel Ruiz I. Altaba et al; micro-injection into drosophila embryos, Philip Ingham and Zandy Forbes; cell transplantation in xenopus, Ariel Ruiz I. Altaba; transplantation in avian embryos, Claudio D. Stern; chick limb buds, Cheryll tickle; nuclear transplantation, John B. Gordon; laser ablation of cells, Judith Eisen et al. Part 3 Cellular techniques: studying cell movements in vivo by time-lapse video microscopy, Peter V. Thorogood and Dee Amanze; induction assays in Xenopus, Ariel Ruiz I. Altaba; culture of avian neural crest cells, Elisabeth Dupin and Nicole M. Le Douarin; embryonic stem cells and gene targeting, Elizabeth J. Robertson and Gail R. Martin; introduction of genes using retroviral vectors, Jack Price. Part 4 Molecular techniques: immonocytochemistry of embryonic material, Claudio D. Stern; RNAase protection assays, Ariel Ruiz I. Altaba; cDNA library construction, Akira Kakizuka et al; analysis of gene expression by reverse transcription polymerase chain reaction, Peter Koopman; cloning genes using the polymerase chain reaction, Peter W.H. Holland; in situ hybridization, David G. Wilkinson. Appendices: stage tables; suppliers of specialist items.
