This book shows the chemist who is already familiar with the basics of HPLC how to develop HPLC separation methods for a wide variety of situations. It focuses mainly on reversed-phase separations of small-molecules, but also addresses development of normal-phase and ion-pair separations. The authors introduce the use of computers to speed and improve method development, covering commercially available HPLC programs.
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
glossary of symbols, index
Maße
Gewicht
ISBN-13
978-0-471-62782-1 (9780471627821)
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Schweitzer Klassifikation
Getting started; basics of separation, mobile phase effects; the role of the column; systematic method development; difficult separations, the use of other separation variables and procedures; gradient elution; special samples and techniques; computer-assisted method development; method development procedures.
