Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.
Rezensionen / Stimmen
This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates. - Doody's Health Sciences Book Review Journal Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs. - Teratology this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there. - TINS Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols. The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols. - The Quarterly Review of Biology This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists. - Journal of Endocrine Genetics
This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates. - Doody's Health Sciences Book Review Journal Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs. - Teratology this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there. - TINS Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols. The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols. - The Quarterly Review of Biology This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists. - Journal of Endocrine Genetics
Reihe
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
Maße
Höhe: 23.5 cm
Breite: 15.5 cm
Gewicht
ISBN-13
978-0-89603-387-0 (9780896033870)
DOI
Schweitzer Klassifikation
Part I. Mouse Embryo
The Mouse as a Developmental Model
Paul T. Sharpe
Culture of Postimplantation Mouse Embryos
Paul Martin and David L. Cockroft
Organ Culture in the Analysis of Tissue Interactions
Irma Thesleff and Carin Sahlberg
Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining
Gillian M. Morriss-Kay
Cell Grafting and Labeling in Postimplantation Mouse Embryos
Gabriel A. Quinlan, Paul A. Trainor, and Patrick P. L. Tam
Production of Transgenic Rodents by the Microinjection of Cloned DNA Into Fertilized One-Celled Eggs
San Ling Si-Hoe and David Murphy
Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells
Anna-Katerina Hadjantonakis, Melinda Pirity, and András Nagy
Gene Trapping in Mouse Embryonic Stem Cells
Jane Brennan and William C. Skarnes
Production and Growth of Conditionally Immortal Cell Lines from the H-2KbtsA58 Transgenic Mouse
Mark Noble
Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos
Jonathan D. Gilthorpe and Peter W. J. Rigby
Application of lacZ Transgenic Mice to Cell Lineage Studies
Paul A. Trainor, Sheila X. Zhou, Maala Parameswaran, Gabriel A. Quinlan, Monica Gordon, Karin Sturm, and Patrick P. L. Tam
Mouse Primordial Germ Cells: Isolation and In Vitro Culture
Patricia A. Labosky and Brigid L. M. Hogan
Part II. Chicken Embryo
The Avian Embryo: An Overview
Ivor Mason
Chick Embryos: Incubation and Isolation
Ivor Mason
New Culture
Amata Hornbruch
Grafting Hensen's Node
Claudio D. Stern
Grafting of Somites
Claudio D. Stern
Manipulating the Chick Embryo: Notochord Grafts
Andrew Lumsden and Susanne Dietrich
Transplantation of Avian Neural Tissue
SarahGuthrie
Grafting of Apical Ridge and Polarizing Region
Cheryl Tickle
Tissue Recombinations in Collagen Gels
Marysia Placzek and Kim Dale
Quail-Chick Chimeras
Marie-Aimée Teillet, Catherine Ziller, and Nicole M. Le Douarin
Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo
Jonathan D. W. Clarke
Part III. Amphibian Embryos
An Overview of Xenopus Development
C. Michael Jones and Jim Smith
Mesoderm Induction Assays
C. Michael Jones and Jim Smith
Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian
Ray Keller, Ann Poznanski, and Tamira Elul
A Method for Generating Transgenic Frog Embryos
Enrique Amaya and Kristen L. Kroll
Axolotl/newt
Malcolm Maden
Part IV. Zebrafish
The Zebrafish: An Overview of Its Early Development
Nigel Holder and Qiling Xu
Small-Scale Marker-Based Screening For Mutations in Zebrafish Development
Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham
Transgenic Zebrafish
Trevor Jowett
Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function
Nigel Holder and Qiling Xu
Retinoids in Nonmammalian Embryos
Malcolm Maden
Part V. Nonvertebrate Chordates
Protochordates
Peter W. H. Holland and Hiroshi Wada
Part VI. Retrovirus
Gene Transfer to the Rodent Embryo by Retroviral Vectors
Grace K. Pavlath and Marla B. Luskin
Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses
Cairine Logan and Philippa Francis-West
Part VII. Molecular Techniques
Subtractive Hybridization and Construction of cDNA Libraries
Bruce Blumberg and Juan Carlos Izpisúa Belmonte
Differential