A Comparative Methods Approach to the Study of Oocytes and Embryos

This volume in the "Advances in Molecular Biology" series compiles late-1990s techniques used for the study of oocytes and embryos of the mouse, Xenopus (a type of frog), and Drosophila (the fruitfly). The methods cover a number of topics, including oocyte and embryo culture; microinjection; "in situ" hybridization; immunohistochemistry; time-lapse microscopy; intracellular ion measurements; and embryonic dissections. The book consolidates different methods and experimental subjects into a unified reference for the increasingly prominent discipline of developmental biology. The comparative perspective encourages readers to re-evaluate and expand their approaches to developmental biological issues in general. This book is intended for graduate students and professionals.